绿藻门鼓藻属中的谷氨酰胺合成酶同工酶
Glutamine Synthetase Isoenzymes in the Green Soil Alga Stichococcus bacillaris Naeg.
机构信息
Department of Botany, University of Toronto, Toronto, Ontario, Canada M5S 1A1.
出版信息
Plant Physiol. 1987 Feb;83(2):259-61. doi: 10.1104/pp.83.2.259.
Abstract
Two forms of glutamine synthetase (GS(1) and GS(2)) have been separated from cells of Stichococcus bacillaris by fast protein liquid chromatography. The activities of the two isoenzymes were influenced by the composition of the media employed; thiol reagents were essential for stabilizing GS(2) but they suppressed GS(1) activity. The activity of each isoenzyme was, therefore, determined following separate purification procedures. Growth conditions influenced both isoenzymes; GS(2) showed maximum activity under photoautotrophic conditions, whereas GS(1) showed maximum activity under heterotrophic conditions.
摘要
两种形式的谷氨酰胺合成酶（GS(1)和 GS(2)）已通过快速蛋白质液相色谱从聚球藻细胞中分离出来。两种同工酶的活性受到所使用的培养基组成的影响；巯基试剂对稳定 GS(2)是必需的，但它们抑制 GS(1)的活性。因此，每种同工酶的活性都是通过单独的纯化程序来确定的。生长条件影响两种同工酶；GS(2)在光照自养条件下表现出最大活性，而 GS(1)在异养条件下表现出最大活性。
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