Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104.
Plant Physiol. 1987 Mar;83(3):525-8. doi: 10.1104/pp.83.3.525.
A pectin-enriched soluble cell wall fraction (CWF) prepared from suspension cultured tomato cells inhibits binding of Agrobacterium tumefaciens to these cells. It was hypothesized that the CWF contains the plant surface binding site for A. tumefaciens (NT Neff, AN Binns 1985 Plant Physiol 77: 35-42). Experiments described here demonstrate that tomato CWF inhibited tumor formation on potato slices and Agrobacterium binding to intact tomato cells in a dose-dependent fashion. Boiling the fraction reduced both its binding and tumor inhibitory activities. Tumor inhibitory activity was titrated out by increased concentrations of bacterial inocula with no inhibition apparent at 1 x 10(8) bacteria per milliliter. These results indicate that a tomato CWF is enriched for a putative A. tumefaciens binding site which may also be involved in tumor formation in potato.
一种富含果胶的悬浮培养番茄细胞的可溶性细胞壁部分（CWF）可抑制根瘤农杆菌与这些细胞的结合。据推测，CWF 中含有植物表面与根瘤农杆菌结合的位点（NT Neff，AN Binns 1985 年《植物生理学》77：35-42）。这里描述的实验表明，番茄 CWF 以剂量依赖的方式抑制了马铃薯切片上的肿瘤形成和完整番茄细胞上的农杆菌结合。煮沸该部分会降低其结合和肿瘤抑制活性。肿瘤抑制活性可通过增加细菌接种物的浓度滴定出来，在每毫升 1 x 10(8)个细菌时没有明显的抑制作用。这些结果表明，番茄 CWF 富含一种假定的根瘤农杆菌结合位点，该位点可能也参与了马铃薯中的肿瘤形成。
