• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

电穿孔胡萝卜原生质体中异源启动子的表达。

Expression from heterologous promoters in electroporated carrot protoplasts.

作者信息

Boston R S, Becwar M R, Ryan R D, Goldsbrough P B, Larkins B A, Hodges T K

机构信息

Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana 47907.

出版信息

Plant Physiol. 1987 Apr;83(4):742-6. doi: 10.1104/pp.83.4.742.

DOI:10.1104/pp.83.4.742
PMID:16665331
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1056442/
Abstract

Plasmids were constructed that contained promoters of ;plant' genes fused to the bacterial gene for chloramphenicol acetyl transferase. The promoters were isolated from a developmentally regulated Zea mays seed storage protein gene and from the mannopine synthase gene of the octopine type Ti plasmid of Agrobacterium tumefaciens which is constitutively expressed in crown gall tumors. These plasmids were introduced into carrot protoplasts made permeable by electroporation. Expression of chloramphenicol acetyl transferase activity directed by both promoters was positively correlated with DNA concentration. The efficiency of gene transfer was increased by inclusion of polyethylene glycol and by optimization of the voltage used in electroporation.

摘要

构建了质粒，这些质粒含有与氯霉素乙酰转移酶的细菌基因融合的“植物”基因启动子。这些启动子分别从一个受发育调控的玉米种子贮藏蛋白基因以及根癌土壤杆菌章鱼碱型Ti质粒的甘露碱合成酶基因中分离得到，后者在冠瘿瘤中组成型表达。通过电穿孔使胡萝卜原生质体具有通透性后，将这些质粒导入其中。两个启动子指导的氯霉素乙酰转移酶活性的表达与DNA浓度呈正相关。通过加入聚乙二醇以及优化电穿孔所用电压，提高了基因转移效率。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6fc9/1056442/f1047a494d53/plntphys00612-0035-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6fc9/1056442/d413690d91f2/plntphys00612-0033-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6fc9/1056442/ea14a1734a3b/plntphys00612-0034-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6fc9/1056442/f1047a494d53/plntphys00612-0035-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6fc9/1056442/d413690d91f2/plntphys00612-0033-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6fc9/1056442/ea14a1734a3b/plntphys00612-0034-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6fc9/1056442/f1047a494d53/plntphys00612-0035-a.jpg

相似文献

1
Expression from heterologous promoters in electroporated carrot protoplasts.电穿孔胡萝卜原生质体中异源启动子的表达。
Plant Physiol. 1987 Apr;83(4):742-6. doi: 10.1104/pp.83.4.742.
2
Octopine synthase mRNA isolated from sunflower crown gall callus is homologous to the Ti plasmid of Agrobacterium tumefaciens.从向日葵冠瘿组织中分离出的瓜氨酸合酶 mRNA 与根癌农杆菌 Ti 质粒同源。
Proc Natl Acad Sci U S A. 1982 Jan;79(1):86-90. doi: 10.1073/pnas.79.1.86.
3
A T-DNA gene required for agropine biosynthesis by transformed plants is functionally and evolutionarily related to a Ti plasmid gene required for catabolism of agropine by Agrobacterium strains.转化植物合成农杆碱所需的一个T-DNA基因，在功能和进化上与根癌土壤杆菌菌株分解农杆碱所需的一个Ti质粒基因相关。
J Bacteriol. 1997 Aug;179(15):4831-40. doi: 10.1128/jb.179.15.4831-4840.1997.
4
Linear DNA introduced into carrot protoplasts by electroporation undergoes ligation and recircularization.通过电穿孔导入胡萝卜原生质体的线性DNA会发生连接和环化。
Plant Mol Biol. 1990 Jun;14(6):899-908. doi: 10.1007/BF00019388.
5
Octopine-type Ti plasmids code for a mannopine-inducible dominant-negative allele of traR, the quorum-sensing activator that regulates Ti plasmid conjugal transfer.章鱼碱型Ti质粒编码一种受甘露碱诱导的traR显性负等位基因，traR是调控Ti质粒接合转移的群体感应激活因子。
Mol Microbiol. 1998 Jan;27(2):277-88. doi: 10.1046/j.1365-2958.1998.00671.x.
6
Ti plasmid-encoded genes responsible for catabolism of the crown gall opine mannopine by Agrobacterium tumefaciens are homologs of the T-region genes responsible for synthesis of this opine by the plant tumor.根癌土壤杆菌中负责冠瘿碱甘露碱分解代谢的Ti质粒编码基因，是植物肿瘤中负责该冠瘿碱合成的T区域基因的同源物。
J Bacteriol. 1996 Jun;178(11):3275-84. doi: 10.1128/jb.178.11.3275-3284.1996.
7
Highly tumorigenic Agrobacterium tumefaciens strain from crown gall tumors of chrysanthemum.来自菊花冠瘿瘤的高致瘤性根癌土壤杆菌菌株。
Arch Microbiol. 2000 Apr;173(4):311-5. doi: 10.1007/s002030000146.
8
Multiple copies of virG enhance the transient transformation of celery, carrot and rice tissues by Agrobacterium tumefaciens.virG的多个拷贝增强了根癌农杆菌对芹菜、胡萝卜和水稻组织的瞬时转化。
Plant Mol Biol. 1992 Dec;20(6):1071-87. doi: 10.1007/BF00028894.
9
Transfer, maintenance, and expression of bacterial Ti-plasmid DNA in plant cells transformed with A. tumefaciens.根癌土壤杆菌转化的植物细胞中细菌Ti质粒DNA的转移、维持及表达。
Brookhaven Symp Biol. 1977(29):36-49.
10
Coordinated regulation of octopine degradation and conjugative transfer of Ti plasmids in Agrobacterium tumefaciens: evidence for a common regulatory gene and separate operons.根癌土壤杆菌中章鱼碱降解与Ti质粒接合转移的协同调控：存在共同调控基因和独立操纵子的证据
J Bacteriol. 1978 Nov;136(2):775-85. doi: 10.1128/jb.136.2.775-785.1978.

引用本文的文献

1
Carrot cells: a pioneering platform for biopharmaceuticals production.胡萝卜细胞：生物制药生产的开创性平台。
Mol Biotechnol. 2015 Mar;57(3):219-32. doi: 10.1007/s12033-014-9837-y.
2
Transient gene expression in tobacco protoplasts: II. Comparison of the reporter gene systems for CAT, NPT II, and GUS.烟草原生质体中的瞬时基因表达：II. CAT、NPT II 和 GUS 报告基因系统的比较。
Plant Cell Rep. 1988 Jun;7(4):225-8. doi: 10.1007/BF00272529.
3
Gene transfer by electroporation in betulaceae protoplasts: Alnus incana.柏科原生质体电穿孔法基因转移：银桦。

本文引用的文献

1
Inheritance of functional foreign genes in plants.植物中外源基因的功能遗传。
Science. 1984 Feb 3;223(4635):496-8. doi: 10.1126/science.223.4635.496.
2
Turbidimetric measurement of plant cell culture growth.浊度法测量植物细胞培养物的生长。
Plant Physiol. 1976 Mar;57(3):460-2. doi: 10.1104/pp.57.3.460.
3
Direct gene transfer to plants.直接基因转移到植物。
Plant Cell Rep. 1988 Oct;7(6):367-70. doi: 10.1007/BF00269514.
4
Direct analysis of RNA transcripts in electroporated carrot protoplasts.电穿孔胡萝卜原生质体中 RNA 转录物的直接分析。
Plant Cell Rep. 1990 Jul;9(3):129-32. doi: 10.1007/BF00232087.
5
Stress responses in alfalfa (Medicago sativa L.) IV. Expression of defense gene constructs in electroporated suspension cell protoplasts.紫花苜蓿（Medicago sativa L.）的应激反应 IV. 电穿孔悬浮细胞原生质体中防御基因构建体的表达。
Plant Cell Rep. 1990 Jun;9(1):42-6. doi: 10.1007/BF00232133.
6
Factors affecting transient gene expression in electroporated black spruce (Picea mariana) and jack pine (Pinus banksiana) protoplasts.影响电穿孔黑云杉（Picea mariana）和短叶松（Pinus banksiana）原生质体瞬时基因表达的因素。
Theor Appl Genet. 1989 Oct;78(4):531-6. doi: 10.1007/BF00290838.
7
Avocado fruit protoplasts: a cellular model system for ripening studies.鳄梨果实原生质体：一种用于成熟研究的细胞模型系统。
Plant Cell Rep. 1991 Dec;10(10):512-6. doi: 10.1007/BF00234584.
8
Electroporated protoplasts express seed specific gene promoters.电穿孔原生质体表达种子特异性基因启动子。
Plant Cell Rep. 1991 Mar;9(11):602-6. doi: 10.1007/BF00231797.
9
Transient gene expression in electroporated banana (Musa spp., cv. 'Bluggoe', ABB group) protoplasts isolated from regenerable embryogenetic cell suspensions.电穿孔香蕉（Musa spp.，cv. 'Bluggoe'，ABB 群）原生质体中转基因的瞬时表达，这些原生质体是从可再生胚胎发生细胞悬浮液中分离出来的。
Plant Cell Rep. 1994 Feb;13(5):262-6. doi: 10.1007/BF00233316.
10
Deletion of DNA sequences flanking an Mr 19,000 zein gene reduces its transcriptional activity in heterologous plant tissues.删除19000道尔顿玉米醇溶蛋白基因侧翼的DNA序列会降低其在异源植物组织中的转录活性。
Mol Gen Genet. 1988 Feb;211(2):202-9. doi: 10.1007/BF00330595.
EMBO J. 1984 Dec 1;3(12):2717-22. doi: 10.1002/j.1460-2075.1984.tb02201.x.
4
Cloning and sequence analysis reveal structural variation among related zein genes in maize.克隆与序列分析揭示了玉米中相关醇溶蛋白基因间的结构变异。
Cell. 1982 Jul;29(3):1015-26. doi: 10.1016/0092-8674(82)90465-2.
5
Electric field-induced cell-to-cell fusion.电场诱导的细胞间融合。
J Membr Biol. 1982;67(3):165-82. doi: 10.1007/BF01868659.
6
Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.在哺乳动物细胞中表达氯霉素乙酰转移酶的重组基因组。
Mol Cell Biol. 1982 Sep;2(9):1044-51. doi: 10.1128/mcb.2.9.1044-1051.1982.
7
E1A control of gene expression is mediated by sequences 5' to the transcriptional starts of the early viral genes.E1A对基因表达的调控是由早期病毒基因转录起始位点上游5'端的序列介导的。
Mol Cell Biol. 1983 Jul;3(7):1222-34. doi: 10.1128/mcb.3.7.1222-1234.1983.
8
Enhancer-dependent expression of human kappa immunoglobulin genes introduced into mouse pre-B lymphocytes by electroporation.通过电穿孔导入小鼠前B淋巴细胞的人κ免疫球蛋白基因的增强子依赖性表达。
Proc Natl Acad Sci U S A. 1984 Nov;81(22):7161-5. doi: 10.1073/pnas.81.22.7161.
9
Gene transfer into mouse lyoma cells by electroporation in high electric fields.通过在高电场中进行电穿孔将基因导入小鼠淋巴瘤细胞。
EMBO J. 1982;1(7):841-5. doi: 10.1002/j.1460-2075.1982.tb01257.x.
10
Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.氯霉素存在下大肠杆菌中Col E 1质粒复制的性质
J Bacteriol. 1972 May;110(2):667-76. doi: 10.1128/jb.110.2.667-676.1972.