Murakami T, Niwa M, Tokunaga F, Miyata T, Iwanaga S
Department of Virology, Osaka City Institute of Public Health and Environmental Sciences, Japan.
Chemotherapy. 1991;37(5):327-34. doi: 10.1159/000238875.
Direct virus inactivation of tachyplesin I and related isopeptides, which are antimicrobial peptides isolated from the hemocytes of the horseshoe crab (Tachypleus tridentatus and Limulus polyphemus), was examined against several viruses. Vesicular stomatitis virus (VSV) was inactivated by incubation with tachyplesin I and its isopeptides. Influenza A (H1N1) virus was slightly inactivated by tachyplesin I, whereas herpes simplex virus 1 and 2, adenovirus 1, reovirus 2 and poliovirus 1 were resistant to inactivation. The inactivation of VSV by tachyplesin I depended on the concentration, the time and the temperature of incubation. Pretreatment of tachyplesin I with trypsin or lipopolysaccharide of gram-negative bacteria entirely abolished the antiviral activity. Electron microscopy of VSV treated with tachyplesin I showed naked and damaged virions. These data suggest that tachyplesin I directly inactivates the VSV by destroying its envelope subunits.
对从鲎（中国鲎和美洲鲎）血细胞中分离得到的抗菌肽鲎素I及相关异肽进行了针对多种病毒的直接病毒灭活研究。水泡性口炎病毒（VSV）与鲎素I及其异肽孵育后被灭活。甲型流感病毒（H1N1）被鲎素I轻微灭活，而单纯疱疹病毒1型和2型、腺病毒1型、呼肠孤病毒2型和脊髓灰质炎病毒1型对灭活具有抗性。鲎素I对VSV的灭活作用取决于孵育的浓度、时间和温度。用胰蛋白酶或革兰氏阴性菌的脂多糖预处理鲎素I会完全消除其抗病毒活性。用鲎素I处理的VSV的电子显微镜观察显示病毒粒子裸露且受损。这些数据表明鲎素I通过破坏其包膜亚基直接灭活VSV。
