Different neuropeptide Y receptor subtypes in rat and rabbit vas deferens.

Prejunctional neuropeptide Y (NPY) receptors that inhibit the contractions evoked in rat and rabbit vas deferens by field stimulation were investigated by using NPY, [Leu31,Pro34]NPY and the fragments, NPY-(13-36) and NPY-(18-36). NPY, and especially [Leu31,Pro34]NPY, were more potent agonists on the twitch response of the rabbit vas deferens. In contrast the NPY C-terminal fragments, NPY-(13-36) and NPY-(18-36), inhibited the twitch response at lower concentrations in the rat vas deferens. These results indicate that distinct NPY receptor subtypes mediate the biological effect in these two tissues. We suggest that prejunctional receptors in the rat vas deferens are of the Y2-subtype and those in rabbit vas deferens of the Y1-subtype.