β-葡糖苷激活绿豆 UDP-葡萄糖：β-葡聚糖合酶：I. 内源性β-连接葡聚糖脂激活剂的鉴定。
beta-Glucoside Activators of Mung Bean UDP-Glucose: beta-Glucan Synthase : I. Identification of an Endogenous beta-Linked Glucolipid Activator.
机构信息
Department of Biological Chemistry, Institute of Life Sciences, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel.
出版信息
Plant Physiol. 1988 Apr;86(4):1099-103. doi: 10.1104/pp.86.4.1099.
Abstract
Heat-stable activators of membranous beta-glucan synthase have been isolated from the supernatant fraction of crude mung bean (Vigna radiata) extracts by DEAE-cellulose and silica-gel chromatography. One of the activators has been partially purified and characterized on the basis of susceptibility to various enzymes and by analysis of the products formed upon total acid hydrolysis, alkaline-methanolysis, and beta-glucosidase digestion. This activator has the characteristics of a 1,2-dioleoyl diglyceride containing beta-linked glucose residue(s) at the C-3 position. When expressed per mole of glucosyl residues, the maximal K(a) value of the activator is estimated to be 25 micromolar. Both the intact glucosyl and fatty acid moiety are essential to the stimulatory effect of the activator.
摘要
已通过 DEAE-纤维素和硅胶层析从粗绿豆（Vigna radiata）提取物的上清液部分中分离出膜结合β-葡聚糖合酶的热稳定激活剂。其中一种激活剂已根据对各种酶的敏感性以及通过分析总酸水解、碱性甲醇解和β-葡萄糖苷酶消化形成的产物进行了部分纯化和表征。这种激活剂具有 1,2-二油酰基二甘油酯的特性，在 C-3 位置含有β-连接的葡萄糖残基(s)。以每摩尔葡萄糖基残基表示，激活剂的最大 K(a)值估计为 25 微摩尔。完整的葡萄糖基和脂肪酸部分对于激活剂的刺激作用都是必不可少的。
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