Linder M C, Moor J R, Munro H N, Morris H P
Biochim Biophys Acta. 1975 Apr 29;386(2):409-21. doi: 10.1016/0005-2795(75)90284-6.
Ferritins purified from several normal and malignant rat tissues were examined for amino acid composition, content of tryptic peptides, available sulfhydryl groups and subunit sizes and proportion. Ferritin extracted from adult kidney, neonatal liver and hepatic and renal tumors differed from the ferritin of adult rat liver in migration on electrophoretic gels and in antibody affinity, but did not differ among themselves. Nevertheless, they showed distinctive differences in amino acid composition and tryptic peptide content. All of them and also adult liver ferritin contained two major species of subunits differing in molecular weight. The proportions of subunits, and the available sulfhydryl groups of the intact ferritin molecules, differed among these tissue ferritins. On the basis of amino acid and peptide content, the ferritins of hepatomas and the renal tumor analyzed showec the greatest similarity but not identity. The ferritin of neonatal liver was next most similar. Kidney ferritin differed considerably in composition from tumor and neonatal ferritins, while adult liver ferritin was the most extremely divergent of the series examined. A similar progressive difference was found on examining the proportions of subunits and sulfhydryl groups in these ferritins. However, changes in subunit proportion cannot explain the amino acid and peptide compositional changes.
对从几只正常和恶性大鼠组织中纯化得到的铁蛋白进行了氨基酸组成、胰蛋白酶肽含量、可用巯基基团以及亚基大小和比例的检测。从成年肾脏、新生肝脏以及肝脏和肾脏肿瘤中提取的铁蛋白，在电泳凝胶上的迁移情况和抗体亲和力方面与成年大鼠肝脏的铁蛋白不同，但它们彼此之间并无差异。然而，它们在氨基酸组成和胰蛋白酶肽含量上显示出明显差异。所有这些铁蛋白以及成年肝脏铁蛋白都含有两种分子量不同的主要亚基。这些组织铁蛋白的亚基比例以及完整铁蛋白分子的可用巯基基团各不相同。基于氨基酸和肽的含量，所分析的肝癌和肾肿瘤的铁蛋白显示出最大的相似性，但并非完全相同。新生肝脏的铁蛋白次之。肾脏铁蛋白在组成上与肿瘤和新生铁蛋白有很大差异，而成年肝脏铁蛋白在所检测的系列中差异最大。在检测这些铁蛋白中亚基比例和巯基基团时也发现了类似的逐步差异。然而，亚基比例的变化无法解释氨基酸和肽组成的变化。
