过量表达人肿瘤坏死因子-α突变体并形成包涵体的重组大肠杆菌细胞的电子显微镜观察
Electron microscopic observation of recombinant Escherichia coli cells overproducing human tumor necrosis factor-alpha mutant as inclusion bodies.
作者信息
Moriya H, Kaneko J, Nakamura S, Osumi M
机构信息
Material Analysis Research Center, Teijin Limited, Tokyo, Japan.
出版信息
J Electron Microsc (Tokyo). 1991 Oct;40(5):364-7.
Escherichia coli C600 r-m- carrying plasmid pTNF483 (E. coli [pTNF483]) produces a tumor necrosis factor-alpha (TNF-alpha) mutant protein in an insoluble form. A swollen region was observed in the SEM images to encircle the outside of most of the E. coli [pTNF483] cells just like a bandage. On the other hand, inclusion bodies of the TNF-alpha mutant as large as the short axis of the cell were observed in TEM images. This position was regarded as coinciding with the swollen region of SEM images. The inclusion bodies revealed in the swollen region of the cell envelopes were clearly observed in SEM images of isolated insoluble structures obtained by centrifugal sedimentation of a sonicated cell suspension.
携带质粒pTNF483的大肠杆菌C600 r-m-（大肠杆菌[pTNF483]）产生不溶性形式的肿瘤坏死因子-α（TNF-α）突变蛋白。在扫描电子显微镜（SEM）图像中观察到一个肿胀区域，就像绷带一样环绕着大多数大肠杆菌[pTNF483]细胞的外部。另一方面，在透射电子显微镜（TEM）图像中观察到与细胞短轴一样大的TNF-α突变体包涵体。该位置被认为与SEM图像中的肿胀区域一致。在通过超声处理的细胞悬液离心沉淀获得的分离不溶性结构的SEM图像中，可以清楚地观察到在细胞包膜肿胀区域中显示的包涵体。
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