Nakamura K, Takahashi T, Tsuyuoka R, Ueda Y, Suzuki A, Okuno Y, Ihara Y, Seko S, Okada T, Kumagai N
College of Medical Technology, Kyoto University.
Jpn J Clin Oncol. 1991 Dec;21(6):395-9.
We have tried to demonstrate and identify colony-stimulating factor (CSF) activity in the plasma, pleural fluid, ascites or culture supernatant of tumor cells in 11 patients with malignant tumors associated with unexplained persistent leukocytosis. The specimens were treated with anti-granulocyte (G)-CSF or anti-granulocyte/macrophage (GM)-CSF monoclonal antibodies, then added to GM-progenitor (CFU-GM) cultures without exogenous CSFs. In all patients, untreated specimens generated CFU-GM-derived colonies, and colony formation was clearly inhibited by only one of the two antibodies, indicating the presence of either G-CSF or GM-CSF in the specimens. Furthermore, we measured the concentrations of G-CSF or GM-CSF in the specimens using an enzyme-linked immunosorbent assay, and confirmed the results by CFU-GM assay. Two patients were shown to have GM-CSF-producing tumors, while the other patients were G-CSF-producing. These assays are useful in identifying CSF activity in patients with CSF-producing tumors.
我们试图在11例伴有无法解释的持续性白细胞增多的恶性肿瘤患者的血浆、胸水、腹水或肿瘤细胞培养上清液中证明并鉴定集落刺激因子（CSF）活性。将标本用抗粒细胞（G）-CSF或抗粒细胞/巨噬细胞（GM）-CSF单克隆抗体处理，然后添加到无外源性CSF的GM祖细胞（CFU-GM）培养物中。在所有患者中，未经处理的标本产生了CFU-GM衍生集落，并且集落形成仅被两种抗体中的一种明显抑制，表明标本中存在G-CSF或GM-CSF。此外，我们使用酶联免疫吸附测定法测量了标本中G-CSF或GM-CSF的浓度，并通过CFU-GM测定法证实了结果。两名患者被证明有产生GM-CSF的肿瘤，而其他患者则产生G-CSF。这些测定法有助于鉴定产生CSF的肿瘤患者中的CSF活性。
