Department of Food Science and Nutrition, The Ohio Agricultural Research and Development Center, The Ohio State University, Columbus, Ohio 43210.
Plant Physiol. 1989 Sep;91(1):148-50. doi: 10.1104/pp.91.1.148.
Kinetic parameters for cytokinin nucleosidase activity which catalyzes the deribosylation of N(6)(Delta(2)-isopentenyl)adenosine (I(6)Ado) to produce the more "active" free base N(5)(Delta(2)-isopenetyl)adenine (I(6)Ade) were compared for a normally ripening tomato (Lycopersicon esculentum L.) cultivar Rutgers, and two mutant tomato varieties (Nor and Rin). K(m) for nucleosidase activity in Rutgers was lower (K(m) = 0.1 millimolar) than that in either Nor (K(m) = 0.14 millimolar) or Rin (K(m) = 0.13 millimolar).
细胞分裂素核苷酶活性的动力学参数,该酶催化 N(6)(Δ(2)-异戊烯基)腺苷(I(6)Ado)的去核糖基化,生成更“活跃”的游离碱基 N(5)(Δ(2)-异戊烯基)腺嘌呤(I(6)Ade),用于比较正常成熟的番茄(Lycopersicon esculentum L.)品种 Rutgers 以及两种突变番茄品种(Nor 和 Rin)。Rutgers 中核苷酶活性的 K(m)较低(K(m) = 0.1 毫摩尔),低于 Nor(K(m) = 0.14 毫摩尔)或 Rin(K(m) = 0.13 毫摩尔)。