College of Forest, Nanjing Forestry University, Nanjing 210037, China.
College of Horticulture, Jinling Institute of Technology, Nanjing 210038, China;
Int J Mol Sci. 2018 Mar 26;19(4):983. doi: 10.3390/ijms19040983.
miRNAs are important regulators of plant gene expression. To better characterize their functions, we applied high-throughput sequencing and degradome analyses to investigate three blueberry () tissues. A total of 127 known and 101 novel miRNAs were identified. Moreover, 141 targets for 42 known and 19 novel miRNAs were experimentally validated by degradome sequencing. A functional analysis of these miRNA targets revealed they were associated with diverse biological activities and several pathways, e.g., anthocyanin biosynthesis and cytokinin signal transduction. The data presented herein expand our understanding of the regulation of blueberry miRNAs during floral and fruit development stages. They may also provide new insights into the roles of miRNAs during anthocyanin biosynthesis in blueberry fruits.
miRNAs 是植物基因表达的重要调控因子。为了更好地描述它们的功能，我们应用高通量测序和降解组分析研究了三种蓝莓组织。共鉴定出 127 个已知和 101 个新的 miRNA。此外，通过降解组测序实验验证了 42 个已知和 19 个新的 miRNA 的 141 个靶标。对这些 miRNA 靶标的功能分析表明，它们与多种生物活性和几个途径有关，如类黄酮生物合成和细胞分裂素信号转导。本文提供的这些数据扩展了我们对蓝莓花和果实发育阶段 miRNA 调控的理解。它们还可能为 miRNA 在蓝莓果实中类黄酮生物合成过程中的作用提供新的见解。
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