Fawley M W
Department of Botany, North Dakota State University, Fargo, North Dakota 58105.
Plant Physiol. 1989 Oct;91(2):727-32. doi: 10.1104/pp.91.2.727.
A new form of chlorophyll c has been isolated from the pyrmnesiophyte Pavlova gyrans Butcher. This pigment is spectrally similar to chlorophyll c(2), but all the absorption maxima (454, 583, and 630 nm in diethyl ether) are shifted 4 to 6 nanometers to longer wavelengths. The new pigment can be separated from other chlorophyll c-type pigments by reversed-phase high performance liquid chromatography and thin layer chromatography. Both chlorophylls c(1) and c(2) are found with the new chlorophyll c pigment in P. gyrans, and it has also been detected in the chrysophyte Synura petersenii Korsh. The light-harvesting function of the new chlorophyll c pigment is indicated by its presence along with chlorophyll c(1) and c(2) in a light-harvesting pigment-protein complex isolated from P. gyrans in which chlorophyll c pigments efficiently transfer absorbed light energy to chlorophyll a.
一种新形式的叶绿素c已从定鞭藻陀螺帕夫藻(Pavlova gyrans Butcher)中分离出来。这种色素在光谱上与叶绿素c(2)相似,但所有吸收峰(在乙醚中为454、583和630纳米)都向更长波长方向移动了4至6纳米。这种新色素可通过反相高效液相色谱法和薄层色谱法与其他叶绿素c型色素分离。在陀螺帕夫藻中,叶绿素c(1)和c(2)都与这种新的叶绿素c色素共存,并且在金藻彼得森合尾藻(Synura petersenii Korsh)中也检测到了它。从陀螺帕夫藻中分离出的一种捕光色素 - 蛋白质复合物中,新的叶绿素c色素与叶绿素c(1)和c(2)一起存在,这表明了它的捕光功能,在该复合物中,叶绿素c色素能有效地将吸收的光能传递给叶绿素a。