Barrett J, Anderson J M
Biochim Biophys Acta. 1980 May 9;590(3):309-23. doi: 10.1016/0005-2728(80)90202-9.
Acrocarpia paniculata thylakoids were fragmented with Triton X-100 and the pigment-protein complexes so released were isolated by sucrose density gradient centrifugation. Three main chlorophyll-carotenoid-protein complexes with distinct pigment compositions were isolated. (1) A P-700-chlorophyll a-protein complex, with a ratio of 1 P-700: 38 chlorophyll a: 4 beta-carotene molecules, had similar absorption and fluorescence characteristics to the chlorophyll-protein complex 1 isolated with Triton X-100 from higher plants, green algae and Ecklonia radiata. (2) an orange-brown complex had a chlorophyll a : c2 : fucoxanthin molar ratio of 2 : 1 : 2. this complex had no chlorophyll c1 and contained most of the fucoxanthin present in the chloroplasts. This pigment complex is postulated to be the main light-harvesting complex of brown seaweeds. (3) A green complex had a chlorophyll a : c1 : c2 : violaxanthin molar ratio of 8 : 1 : 1. This also is a light-harvesting complex. the absorption and fluorescence spectral characteristics and other physical properties were consistent with the pigments of these three major complexes being bound to protein. Differential extraction of brown algal thylakoids with Triton X-100 showed that a chlorophyll c2-fucoxanthin-protein complex was a minor pigment complex of these thylakoids.
用Triton X - 100使圆锥顶孢藻的类囊体破碎，通过蔗糖密度梯度离心法分离如此释放出的色素 - 蛋白质复合物。分离出了三种主要的具有不同色素组成的叶绿素 - 类胡萝卜素 - 蛋白质复合物。（1）一种P - 700 - 叶绿素a - 蛋白质复合物，其比例为1个P - 700：38个叶绿素a：4个β - 胡萝卜素分子，其吸收和荧光特性与用Triton X - 100从高等植物、绿藻和辐射马尾藻中分离出的叶绿素 - 蛋白质复合物1相似。（2）一种橙棕色复合物，其叶绿素a：c2：岩藻黄质的摩尔比为2：1：2。该复合物不含叶绿素c1，且包含叶绿体中存在的大部分岩藻黄质。推测这种色素复合物是褐藻的主要捕光复合物。（3）一种绿色复合物，其叶绿素a：c1：c2：紫黄质的摩尔比为8：1：1。这也是一种捕光复合物。这三种主要复合物的色素与蛋白质结合后的吸收和荧光光谱特性以及其他物理性质是一致的。用Triton X - 100对褐藻类囊体进行差异提取表明，叶绿素c2 - 岩藻黄质 - 蛋白质复合物是这些类囊体的一种次要色素复合物。
