U.S. Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Plant Sciences Institute, Beltsville, Maryland 20705.
Plant Physiol. 1990 Feb;92(2):427-33. doi: 10.1104/pp.92.2.427.
The effect of alcohols which stimulate or have no effect on germination on the composition and synthetic pattern of proteins in the cellular membranes of Echinochloa crus-galli (L.) Beauv. seeds was studied. Imbibition of dry seeds was accompanied by an increase in the synthesis of proteins and by synthesis of new proteins in their intracellular membranes. The transition of the seeds from a dormant to a nondormant state was associated with synthesis of specific proteins and a decrease in content of others in the plasma membrane. The synthesis of a 23 kilodalton protein was strongly increased upon release from dormancy. The changes in the pattern of protein synthesis were not directly associated with the beginning of germination. The results suggest that the plasma membrane constitutes the first site in the seed cells, at which the stimulus from external factors affecting seed dormancy is detected.
研究了刺激或不刺激发芽的醇类对稗草（Echinochloa crus-galli（L.）Beauv.）种子细胞内膜组成和蛋白质合成模式的影响。干种子的吸胀伴随着蛋白质合成的增加和细胞内膜中新蛋白质的合成。种子从休眠状态向非休眠状态的转变与特定蛋白质的合成和质膜中其他蛋白质含量的减少有关。从休眠中释放出来后，23 千道尔顿蛋白的合成大大增加。蛋白质合成模式的变化与萌发的开始没有直接关系。结果表明，质膜构成了种子细胞中第一个检测到影响种子休眠的外部因素刺激的部位。
