一种可水解甘蔗汁中天然多糖的果聚糖酶的纯化及部分特性分析
Purification and Partial Characterization of a Fructanase which Hydrolyzes Natural Polysaccharides from Sugarcane Juice.
作者信息
Legaz M E, Martin L, Pedrosa M M, Vicente C, de Armas R, Martínez M, Medina I, Rodriguez C W
机构信息
Laboratory of Plant Physiology, Faculty of Biology, Complutense University, 28040 Madrid, Spain.
出版信息
Plant Physiol. 1990 Mar;92(3):679-83. doi: 10.1104/pp.92.3.679.
A new sugarcane (Saccharum officinarum L.) fructanase which hydrolyzes both high molecular weight polysaccharides mid R:Fructose(4):Galactitol(5)mid R:(n) (SP) and moderate-sized carbohydrates mid R:Fructose(2):Galactitol(3)3mid R:(n) (MMWC) has been purified from sugarcane juice. The K(m) value has been estimated to be 33.7 micrograms per milliliter and 20 micrograms per milliliter for SP and MMWC, respectively. The optimal pH and temperature values are 6.0 and 30 degrees C, respectively. Purified protein has a pl value of 6.35 and a molecular weight of 13.2 kilodaltons. Fructanase activity appears to be Mn(2+)-dependent.
一种新的甘蔗（Saccharum officinarum L.）果聚糖酶已从甘蔗汁中纯化出来，该酶既能水解高分子量多糖R：果糖(4)：半乳糖醇(5)R：(n)（SP），也能水解中等大小的碳水化合物R：果糖(2)：半乳糖醇(3)3R：(n)（MMWC）。SP和MMWC的米氏常数（K(m)）值分别估计为每毫升33.7微克和每毫升20微克。最佳pH值和温度值分别为6.0和30摄氏度。纯化后的蛋白质的等电点（pI）值为6.35，分子量为13.2千道尔顿。果聚糖酶活性似乎依赖于锰离子（Mn(2+)）。
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