Mason C B, Manuel L J, Moroney J V
Department of Botany, Louisiana State University, Baton Rouge, Louisiana 70803.
Plant Physiol. 1990 Jun;93(2):833-6. doi: 10.1104/pp.93.2.833.
The biosynthesis of a 36 kilodalton polypeptide of Chlamydomonas reinhardtii was induced by photoautotrophic growth on low CO(2). Fractionation studies using the cell-wall-deficient strain of C. reinhardtii, CC-400, showed that this polypeptide was different from the low CO(2)-induced periplasmic carbonic anhydrase. In addition, the 36 kilodalton polypeptide was found to be localized in intact chloroplasts isolated from low CO(2)-adapting cultures. This protein may, in part, account for the different inorganic carbon uptake characteristics observed in chloroplasts isolated from high and low CO(2)-grown C. reinhardtii cells.
莱茵衣藻36千道尔顿多肽的生物合成是由在低二氧化碳条件下的光合自养生长诱导的。使用莱茵衣藻细胞壁缺陷型菌株CC - 400进行的分级分离研究表明,该多肽与低二氧化碳诱导的周质碳酸酐酶不同。此外,发现36千道尔顿多肽定位于从适应低二氧化碳培养物中分离出的完整叶绿体中。这种蛋白质可能部分解释了从高二氧化碳和低二氧化碳生长的莱茵衣藻细胞中分离出的叶绿体中观察到的不同无机碳吸收特性。