Department of Biochemistry, Michigan State University, East Lansing, Michigan 48824-1319.
Plant Physiol. 1992 Dec;100(4):2113-5. doi: 10.1104/pp.100.4.2113.
Unicellular green algae have a mechanism for concentrating dissolved inorganic carbon (DIC) only when grown in low CO(2). To find proposed transporter protein(s) for DIC, we isolated intact chloroplasts from Dunaliella tertiolecta cells, separated the chloroplast envelopes by isopyknic centrifugation, and separated their polypeptides by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Two peptides of apparent molecular masses of 45 and 47 kD were constituents of the inner chloroplast envelope only if the cells had been adapted to low CO(2) in the light or grown in low CO(2). These two low CO(2)-induced peptides appear to be part of the algal DIC pump.
单细胞绿藻在低 CO2 环境中生长时，有一种浓缩溶解无机碳（DIC）的机制。为了寻找 DIC 的拟转运蛋白，我们从杜氏盐藻细胞中分离完整的叶绿体，通过等密度离心分离叶绿体膜，并用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离其多肽。只有当细胞适应低 CO2 光照或在低 CO2 下生长时，叶绿体膜内的两种多肽（表观分子量分别为 45 和 47 kD）才是组成部分。这两种低 CO2 诱导的多肽似乎是藻类 DIC 泵的一部分。
