Petrie C R, Adams A D, Stamm M, Van Ness J, Watanabe S M, Meyer R B
MicroProbe Corporation, Bothell, Washington 98021.
Bioconjug Chem. 1991 Nov-Dec;2(6):441-6. doi: 10.1021/bc00012a011.
A novel dATP analogue, 3-[5-[(N-biotinyl-6- amiocaproyl)amino]pentyl]-1-(2-deoxy-beta-D-erythro-pentofuranosyl )-1H-pyrazolo[3,4-d]pyrimidin-4-amine 5'-triphosphate (9, bio-13-dAPPTP), which is modified at the 3-position with a flexible linker arm bearing a terminal biotin moiety, has been synthesized. This nucleotide is readily incorporated into DNA probes by nick translation. These probes hybridize to complementary targets as well as probes labeled with bio-dUTP, as judged by slot blot. When incorporated into oligonucleotides, they do not cause the loss of hybridization efficiency that an N-6-substituted adenine nucleotide does when incorporated into the same sites in the oligonucleotide.
一种新型的dATP类似物，即3-[5-[(N-生物素基-6-氨基己酰基)氨基]戊基]-1-(2-脱氧-β-D-赤式-戊呋喃糖基)-1H-吡唑并[3,4-d]嘧啶-4-胺5'-三磷酸酯（9，生物素-13-dAPPTP）已被合成，该类似物在3位用带有末端生物素部分的柔性连接臂进行了修饰。这种核苷酸很容易通过缺口平移掺入DNA探针中。通过狭缝印迹法判断，这些探针与互补靶标杂交的情况与用生物素-dUTP标记的探针相同。当掺入寡核苷酸中时，它们不会像N-6-取代的腺嘌呤核苷酸掺入寡核苷酸相同位点时那样导致杂交效率的损失。
