Department of Biology, University of California, Los Angeles, CA 90024.
Proc Natl Acad Sci U S A. 1986 Apr;83(7):2123-7. doi: 10.1073/pnas.83.7.2123.
We investigated soybean seed protein gene transcription during development. We found that seed protein genes are transcriptionally activated and then repressed during embryogenesis and that these genes are either inactive or transcribed at low levels in the mature plant. We further observed that genes encoding mRNAs with vastly different prevalences are transcribed at similar rates. DNA gel blot studies showed that transcriptionally active and inactive seed protein genes have indistinguishable methylation patterns. We conclude that both transcriptional and posttranscriptional processes regulate seed protein mRNA levels in the absence of detectable DNA methylation changes.
我们研究了大豆种子蛋白基因在发育过程中的转录。我们发现，种子蛋白基因在胚胎发生过程中被转录激活，然后被抑制，而在成熟植物中，这些基因要么不活跃，要么以低水平转录。我们进一步观察到，编码 mRNA 丰度差异很大的基因以相似的速率转录。DNA 凝胶印迹研究表明，转录活跃和不活跃的种子蛋白基因具有相似的甲基化模式。我们的结论是，在没有可检测的 DNA 甲基化变化的情况下，转录和转录后过程共同调节种子蛋白 mRNA 的水平。
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