从柳枝稷叶片提取物中的二甲基烯丙基二磷酸酶促合成异戊二烯。
Enzymatic synthesis of isoprene from dimethylallyl diphosphate in aspen leaf extracts.
机构信息
Department of Chemistry and Biochemistry, and Cooperative Institute for Research in Environmental Sciences, University of Colorado, Boulder, Colorado 80309-0215.
出版信息
Plant Physiol. 1991 Dec;97(4):1588-91. doi: 10.1104/pp.97.4.1588.
Abstract
Aspen (Populus tremuloides Michx.) leaf extracts contain a newly discovered enzyme activity that catalyzes the magnesium ion-dependent elimination of diphosphate from dimethylallyl diphosphate with rearrangement to form isoprene (2-methyl, 1-3-butadiene). This isoprene synthase activity has been partially purified. The nonenzymatic reaction of dimethylallyl diphosphate to isoprene, known to be acid catalyzed, may be insignificant at physiological pH. In contrast, the enzymatic reaction may be responsible for the majority of light-dependent isoprene production by isoprene-emitting plants.
摘要
杨树叶提取物含有一种新发现的酶活性，该酶活性可以催化二甲基烯丙基二磷酸在镁离子依赖性条件下消除焦磷酸，并通过重排形成异戊二烯（2-甲基-1,3-丁二烯）。这种异戊二烯合酶活性已被部分纯化。已知已知非酶促反应，焦磷酸二甲烯丙酯向异戊二烯的反应可能在生理 pH 值下不重要。相比之下，该酶促反应可能是异戊二烯排放植物中光依赖性异戊二烯产生的主要原因。
相似文献
1
Enzymatic synthesis of isoprene from dimethylallyl diphosphate in aspen leaf extracts.从柳枝稷叶片提取物中的二甲基烯丙基二磷酸酶促合成异戊二烯。
Plant Physiol. 1991 Dec;97(4):1588-91. doi: 10.1104/pp.97.4.1588.
2
Characterization of aspen isoprene synthase, an enzyme responsible for leaf isoprene emission to the atmosphere.
J Biol Chem. 1995 Jun 2;270(22):13010-6. doi: 10.1074/jbc.270.22.13010.
3
Isoprene synthase activity parallels fluctuations of isoprene release during growth of Bacillus subtilis.在枯草芽孢杆菌生长过程中，异戊二烯合酶活性与异戊二烯释放的波动情况相似。
Biochem Biophys Res Commun. 2002 May 31;294(1):71-5. doi: 10.1016/S0006-291X(02)00435-7.
4
Biochemical characterization of an isoprene synthase from Campylopus introflexus (heath star moss).弯曲曲柄藓（ Heath star moss）异戊二烯合酶的生化特性
Plant Physiol Biochem. 2015 Sep;94:209-15. doi: 10.1016/j.plaphy.2015.06.008. Epub 2015 Jun 12.
5
Elevated atmospheric CO2 concentration leads to increased whole-plant isoprene emission in hybrid aspen (Populus tremula × Populus tremuloides).大气 CO2 浓度升高会导致杂种欧洲山杨（Populus tremula×Populus tremuloides）的整株植物异戊二烯排放量增加。
New Phytol. 2013 May;198(3):788-800. doi: 10.1111/nph.12200. Epub 2013 Feb 27.
6
Thermotolerance of leaf discs from four isoprene-emitting species is not enhanced by exposure to exogenous isoprene.暴露于外源异戊二烯并不能提高四种释放异戊二烯的植物叶片圆盘的耐热性。
Plant Physiol. 1999 Jul;120(3):821-6. doi: 10.1104/pp.120.3.821.
7
Spectacular Oscillations in Plant Isoprene Emission under Transient Conditions Explain the Enigmatic CO2 Response.瞬态条件下植物异戊二烯排放中的壮观振荡解释了神秘的二氧化碳响应。
Plant Physiol. 2016 Dec;172(4):2275-2285. doi: 10.1104/pp.16.01002. Epub 2016 Oct 21.
8
Relationship of isopentenyl diphosphate (IDP) isomerase activity to isoprene emission of oak leaves.异戊烯基二磷酸（IDP）异构酶活性与橡树叶异戊二烯排放的关系。
Tree Physiol. 2002 Oct;22(14):1011-8. doi: 10.1093/treephys/22.14.1011.
9
Enzymatic synthesis of methylbutenol from dimethylallyl diphosphate in needles of Pinus sabiniana.从加州刺柏针叶中的二甲基烯丙基二磷酸酯酶促合成甲基丁烯醇。
Arch Biochem Biophys. 2000 Nov 1;383(1):128-34. doi: 10.1006/abbi.2000.2051.
10
Two unexpected promiscuous activities of the iron-sulfur protein IspH in production of isoprene and isoamylene.铁硫蛋白IspH在异戊二烯和异戊烯生产中的两种意外的混杂活性。
Microb Cell Fact. 2016 May 11;15:79. doi: 10.1186/s12934-016-0476-9.
引用本文的文献
1
Biosynthetic Machinery to Abiotic Stress-Driven Emission: Decoding Multilayer Regulation of Volatile Terpenoids in Plants.生物合成机制与非生物胁迫驱动的排放：解析植物中挥发性萜类化合物的多层调控
Antioxidants (Basel). 2025 May 31;14(6):673. doi: 10.3390/antiox14060673.
2
Insights into the biotechnology potential of .对……生物技术潜力的洞察。
你提供的原文不完整，请补充完整内容以便我准确翻译。
Front Microbiol. 2022 Dec 15;13:1034674. doi: 10.3389/fmicb.2022.1034674. eCollection 2022.
3
An Overview of the Isoprenoid Emissions From Tropical Plant Species.热带植物物种类异戊二烯排放概述
Front Plant Sci. 2022 May 20;13:833030. doi: 10.3389/fpls.2022.833030. eCollection 2022.
4
MICROBIAL isoprene production: an overview.微生物异戊二烯生产：概述。
World J Microbiol Biotechnol. 2022 May 31;38(7):122. doi: 10.1007/s11274-022-03306-4.
5
Improved plant heat shock resistance is introduced differently by heat and insect infestation: the role of volatile emission traits.热胁迫和虫害以不同的方式提高植物的抗热性：挥发物排放特性的作用。
Oecologia. 2022 May;199(1):53-68. doi: 10.1007/s00442-022-05168-x. Epub 2022 Apr 26.
6
Integrated Four Comparative-Omics Reveals the Mechanism of the Terpenoid Biosynthesis in Two Different Overwintering Phenotypes.整合四种比较组学揭示两种不同越冬表型中萜类生物合成的机制
Front Plant Sci. 2021 Sep 29;12:740755. doi: 10.3389/fpls.2021.740755. eCollection 2021.
7
Isoprene: An Antioxidant Itself or a Molecule with Multiple Regulatory Functions in Plants?异戊二烯：自身是一种抗氧化剂还是在植物中具有多种调节功能的分子？
Antioxidants (Basel). 2021 Apr 27;10(5):684. doi: 10.3390/antiox10050684.
8
Protein expression plasticity contributes to heat and drought tolerance of date palm.蛋白质表达可塑性有助于提高枣椰树的耐热性和耐旱性。
Oecologia. 2021 Dec;197(4):903-919. doi: 10.1007/s00442-021-04907-w. Epub 2021 Apr 21.
9
Source of 12C in Calvin-Benson cycle intermediates and isoprene emitted from plant leaves fed with 13CO2.卡尔文-本森循环中间产物和 13CO2 饲喂的植物叶片中异戊二烯的 12C 来源。
Biochem J. 2020 Sep 18;477(17):3237-3252. doi: 10.1042/BCJ20200480.
10
Isoprene Oxidation by the Gram-Negative Model bacterium sp. WS11.革兰氏阴性模式细菌WS11对异戊二烯的氧化作用
Microorganisms. 2020 Feb 29;8(3):349. doi: 10.3390/microorganisms8030349.
本文引用的文献
1
Redox-modulation of chloroplast enzymes : a common principle for individual control.叶绿体酶的氧化还原调节：个体控制的共同原则。
Plant Physiol. 1991 May;96(1):1-3. doi: 10.1104/pp.96.1.1.
2
Isoprene emission from aspen leaves : influence of environment and relation to photosynthesis and photorespiration.山杨树叶的异戊二烯排放：环境的影响以及与光合作用和光呼吸作用的关系
Plant Physiol. 1989 May;90(1):267-74. doi: 10.1104/pp.90.1.267.
3
Production of isoprene by leaf tissue.叶片组织生产异戊二烯。
Plant Physiol. 1975 Jun;55(6):982-7. doi: 10.1104/pp.55.6.982.
4
Linear one-step assay for the determination of orthophosphate.用于测定正磷酸盐的线性一步分析法。
Anal Biochem. 1983 Jul 15;132(2):254-8. doi: 10.1016/0003-2697(83)90004-0.
5
Acid-catalyzed formation of isoprene from a mevalonate-derived product using a rat liver cytosolic fraction.
J Biol Chem. 1985 Feb 10;260(3):1382-5.
6
Measurement of protein using bicinchoninic acid.使用二辛可宁酸测定蛋白质。
Anal Biochem. 1985 Oct;150(1):76-85. doi: 10.1016/0003-2697(85)90442-7.
7
Synthesis of allylic and homoallylic isoprenoid pyrophosphates.烯丙基和高烯丙基类异戊二烯焦磷酸酯的合成。
Methods Enzymol. 1985;110:130-44. doi: 10.1016/s0076-6879(85)10068-6.
8
A simple colorimetric assay method for pyrophosphate in the presence of a 1000-fold excess of orthophosphate: application of the method to the study of pyrophosphate metabolism in mitochondria.一种在正磷酸盐存在量超过1000倍时用于检测焦磷酸盐的简单比色测定法：该方法在研究线粒体焦磷酸盐代谢中的应用
Anal Biochem. 1989 Feb 1;176(2):390-4. doi: 10.1016/0003-2697(89)90329-1.
9
Preparation of extracts from plants.植物提取物的制备。
Methods Enzymol. 1990;182:174-93. doi: 10.1016/0076-6879(90)82016-u.
10
A continuous spectrophotometric assay for argininosuccinate synthetase based on pyrophosphate formation.
Anal Biochem. 1976 Dec;76(2):423-30. doi: 10.1016/0003-2697(76)90337-7.
Zotero 插件