Fukuda M, Kono T, Ishii M, Hamada T
Department of Dermatology, Osaka City University Medical School, Japan.
Clin Exp Dermatol. 1991 Nov;16(6):407-10. doi: 10.1111/j.1365-2230.1991.tb01223.x.
Herpes simplex viral DNA was detected in biopsy specimens obtained from four patients with Kaposi's varicelliform eruption using an in situ hybridization technique with biotinylated complementary DNA probes. Herpes simplex viral DNA was consistently found almost exclusively in the nuclei of giant cells and balloon cells in vesicles during day 2, 5 and 7 of the disease. In the nuclei, the viral DNA staining pattern was granular or agglomerate. No positive staining was observed in the cytoplasm.
采用生物素化互补DNA探针原位杂交技术，在4例水痘样疹患者的活检标本中检测到单纯疱疹病毒DNA。在疾病的第2天、第5天和第7天，单纯疱疹病毒DNA几乎总是仅在水疱内巨细胞和气球样细胞的细胞核中发现。在细胞核中，病毒DNA染色模式呈颗粒状或凝聚状。在细胞质中未观察到阳性染色。
