Kim S R, Kim Y, Costa M A, An G
Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340.
Plant Physiol. 1992 Apr;98(4):1479-83. doi: 10.1104/pp.98.4.1479.
Effect of salicylic acid (SA) on the expression of the potato proteinase inhibitor (PI) II promoter was studied with transgenic tobacco plants (Nicotiana tabacum) carrying a gene fusion between the PI-II promoter and the chloramphenicol acetyltransferase (cat) reporter. As previously observed, the PI-II promoter was inducible by wounding and the promoter activity was further enhanced by sucrose. Addition of SA did not influence the wound induction of the PI-II promoter but significantly inhibited the sucrose response. The 5'-deletion mutant -573 was unable to respond to wounding but did respond to sucrose and SA. The 3'-deletion analysis indicated the presence of a sucrose-responsive element between -574 and -520. A study of the insertion mutants revealed the function of another sucrose-responsive element between -522 and -500. Enhancer effects of these sucrose-responsive elements were inhibited by SA. These studies suggest that SA inhibits PI-II promoter activity by decreasing the sucrose response. Analysis of SA-related chemicals revealed that only acetyl-SA showed a similar inhibitory effect, and other hydroxybenzoic acids had little or no effect on the sucrose enhancer activity. Therefore, it seems that the interaction between SA and the receptor molecule is specific.
利用携带蛋白酶抑制剂(PI)II启动子与氯霉素乙酰转移酶(cat)报告基因融合基因的转基因烟草植株(烟草),研究了水杨酸(SA)对马铃薯蛋白酶抑制剂(PI)II启动子表达的影响。如先前观察到的,PI-II启动子可被创伤诱导,且启动子活性会因蔗糖而进一步增强。添加SA不影响PI-II启动子的创伤诱导,但显著抑制蔗糖反应。5'-缺失突变体-573对创伤无反应,但对蔗糖和SA有反应。3'-缺失分析表明在-574和-520之间存在一个蔗糖反应元件。对插入突变体的研究揭示了在-522和-500之间另一个蔗糖反应元件的功能。SA抑制了这些蔗糖反应元件的增强子效应。这些研究表明,SA通过降低蔗糖反应来抑制PI-II启动子活性。对SA相关化学物质的分析表明,只有乙酰水杨酸表现出类似的抑制作用,而其他羟基苯甲酸对蔗糖增强子活性几乎没有影响或没有影响。因此,SA与受体分子之间的相互作用似乎是特异性的。