Kim S R, Choi J L, Costa M A, An G
Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340.
Plant Physiol. 1992 Jun;99(2):627-31. doi: 10.1104/pp.99.2.627.
The potato proteinase inhibitor II promoter was studied to identify cis-acting regulatory sequences involved in methyl jasmonate (MJ) response using transgenic tobacco plants carrying various lengths of the promoter fused to a chloramphenicol acetyltransferase reporter gene. An internal fragment between -625 and -520 was sufficient to confer a response to MJ, wounding, or sucrose when it was placed upstream of the nos promoter -101, which contains the CAAT-TATA region. Deletion of the proteinase inhibitor II promoter sequence upstream of -611 did not affect the MJ response, but a further deletion to -573 eliminated the response. The 3'-deletion study showed that the DNA sequence downstream from -520 is dispensable. However, 3'-deletion mutant -574 did not respond to the MJ treatment. These results indicated that an element essential for the MJ response is located at the -574/-573 region where the G-box sequence (CACGTGG) is located. The G-box sequence was not required for the sucrose enhancer effect, suggesting that the MJ response mechanism is different from that of sucrose.
利用携带与氯霉素乙酰转移酶报告基因融合的不同长度启动子的转基因烟草植株，对马铃薯蛋白酶抑制剂II启动子进行了研究，以鉴定参与茉莉酸甲酯（MJ）应答的顺式作用调控序列。当位于包含CAAT - TATA区域的nos启动子 - 101上游时，- 625至 - 520之间的内部片段足以赋予对MJ、创伤或蔗糖的应答。删除 - 611上游的蛋白酶抑制剂II启动子序列不影响MJ应答，但进一步删除至 - 573则消除了应答。3' - 删除研究表明，- 520下游的DNA序列是可有可无的。然而，3' - 删除突变体 - 574对MJ处理无应答。这些结果表明，MJ应答所必需的元件位于 - 574 / - 573区域，即G - 盒序列（CACGTGG）所在的位置。G - 盒序列对于蔗糖增强效应不是必需的，这表明MJ应答机制与蔗糖的不同。
