Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340.
Plant Physiol. 1992 Apr;98(4):1515-7. doi: 10.1104/pp.98.4.1515.
Clomazone, an herbicide that reduces the levels of leaf carotenoids and chlorophylls, is thought to act by inhibiting isopentenyl pyrophosphate isomerase or the prenyltransferases responsible for the synthesis of geranylgeranyl pyrophosphate. Cell-free extracts prepared from the oil glands of common sage (Salvia officinalis) are capable of converting isopentenyl pyrophosphate to geranylgeranyl pyrophosphate. Clomazone at 250 micromolar (a level that produced leaf bleaching) had no detectable effect on the activity of the relevant enzymes (isopentenyl pyrophosphate isomerase and the three prenyltransferases, geranyl, farnesyl, and geranylgeranyl pyrophosphate synthases). Thus, inhibition of geranylgeranyl pyrophosphate biosynthesis does not appear to be the mode of action of this herbicide.
氯苯氧菌胺,一种降低叶片类胡萝卜素和叶绿素水平的除草剂,被认为通过抑制异戊烯焦磷酸异构酶或负责合成香叶基焦磷酸的 prenyltransferases 起作用。从普通鼠尾草(Salvia officinalis)的油腺中制备的无细胞提取物能够将异戊烯焦磷酸转化为香叶基焦磷酸。氯苯氧菌胺在 250 微摩尔(导致叶片漂白的水平)时对相关酶(异戊烯焦磷酸异构酶和三种 prenyltransferases,香叶基、法呢基和香叶基焦磷酸合酶)的活性没有明显影响。因此,抑制香叶基焦磷酸生物合成似乎不是这种除草剂的作用模式。