Institut National de la Recherche Agronomique, Station de Botanique et Pathologie végétale, BP 2078 Antibes-Cedex 06606.
Plant Physiol. 1992 Jul;99(3):843-7. doi: 10.1104/pp.99.3.843.
Cryptogein (CRY), a protein secreted by Phytophthora cryptogea, causes necrosis on tobacco (Nicotiana tabacum) plants at the site of application (the stem or the roots) and also on distant leaves. Autoradiography of plantlets after root absorption of radioiodinated CRY demonstrated a rapid migration of the label to the leaf lamina via the veins. Using an anti-CRY antiserum, a CRY-related antigen was detected in the stem and leaves of CRY-treated plants at a distance from the site of application. This antigen had the same molecular weight as CRY and was detected in the leaves as early as 1 hour after stem treatment, i.e. long before necrosis was detectable. The antigen was also detected in plants inoculated with P. cryptogea. The distant location of the necrosis induced by the fungus or by CRY can be ascribed to the migration of this protein, which is toxic to tobacco cells. It is proposed that CRY, which also elicits defense reactions in tobacco, might contribute to the hypersensitive response of tobacco to P. cryptogea.
cryptogein (CRY)，一种由 Phytophthora cryptogea 分泌的蛋白质，会在烟草（Nicotiana tabacum）植物的施药部位（茎或根）和远处的叶片上引起坏死。放射性碘标记的 CRY 被植物吸收后进行放射自显影，结果表明标记物通过叶脉迅速迁移到叶片的表皮。使用抗 CRY 抗血清，在远离施药部位的 CRY 处理植物的茎和叶中检测到与 CRY 相关的抗原。该抗原与 CRY 具有相同的分子量，并且在茎处理后 1 小时即可在叶片中检测到，即在可检测到坏死之前很久。该抗原也在接种 P. cryptogea 的植物中检测到。真菌或 CRY 引起的坏死的远距离位置可归因于这种对烟草细胞有毒的蛋白质的迁移。据推测，CR 在引发烟草防御反应的同时，也可能导致烟草对 P. cryptogea 的过敏反应。
