Minimo C, Bartels P H, Kim D H, Pfeifer T, Dytch H E, Galera-Davidson H, Bibbo M
Department of Pathology, University of Chicago, Illinois 60637.
Anal Quant Cytol Histol. 1991 Dec;13(6):411-7.
Architectural and histometric features for the objective grading of prostate adenocarcinoma in histologic specimens were analyzed in five cases each of Gleason primary grades 2, 3A, 3B, 3C, 4A and 4B, selected as "typical" for the histopathologic images. Tissue sections from the selected cases were stained by the Feulgen method. Fifteen fields for each grade, for a total of 4,430 glands, were digitized by a video-based microphotometer at low resolution (pixel spacing of 2 microns). Outer and inner outlines of the glandular epithelium were traced manually using a mouse. For each field the number of glands, the gland area, the lumen area, the area of the glandular epithelium and the cribriformity factor were computed. The gland area and its variance proved to be useful indicators for lower-grade lesions, whereas the variance of cribriformity resulted in an excellent grading indicator in the Gleason 3-4 range when cribriform glands were present.
对5例Gleason 1级、3A级、3B级、3C级、4A级和4B级前列腺腺癌组织标本进行客观分级的结构和组织测量学特征分析，这些病例被选为组织病理学图像的“典型”病例。所选病例的组织切片采用福尔根染色法。每个分级取15个视野，共计4430个腺体，通过基于视频的显微光度计以低分辨率（像素间距为2微米）进行数字化处理。使用鼠标手动描绘腺上皮的外部和内部轮廓。计算每个视野的腺体数量、腺体面积、管腔面积、腺上皮面积和筛状因子。腺体面积及其方差被证明是低级别病变的有用指标，而当存在筛状腺体时，筛状因子的方差在Gleason 3-4级范围内是一个出色的分级指标。
