Traub M, Spindler K D, Schmitt H P, Messiha F S, Bär W, Pasi A
Endorphin Laboratories, Institute of Forensic Medicine, University of Zürich, Switzerland.
J Med. 1991;22(6):311-26.
In order to identify opioid receptor proteins, we first ascertained the presence of mu-receptors in membranes of the human cerebral frontal cortex, using binding studies performed with sufentanil. Sufentanil binding was reversed by naloxone and prevented by an anti-idiotypic opioid receptor-specific antibody. This antibody was bound specifically by proteins (molecular weights of 66 and 68 Kda) detected in frontal cortex membrane preparations by sodium dodecyl sulphate-polyacrylamide-gel electrophoresis in combination with western blot analysis. Binding of the same antibody was achieved in dot blots of homologous cerebral and cerebellar cortex preparations.
