3',5'-环磷酸腺苷、3',5'-环磷酸鸟苷及茶碱对大鼠离体灌注肾中肾素分泌的影响。

The effects of cyclic adenosine 3',5'-monophosphate and guanosine 3',5'-monophosphate and theophylline on renin secretion in the isolated perfused kidney of the rat.

Peart W S, Quesada T, Tenyi I

Br J Pharmacol. 1975 May;54(1):55-60. doi: 10.1111/j.1476-5381.1975.tb07409.x.

Abstract

The influence of cyclic adenosine 3',5'-monophosphate (cyclic AMP), cylic guanosine 3',5'-monophosphate (cyclic GMP) and theophylline on renin secretion was examined in the isolated kidney of the rat perfused with Krebs dextran solution. 2. Neither cyclic AMP (10(-6) to 10(-4) M) nor dibytyryl cyclic AMP (10(-5) M) produced an increase in renin secretion. 3. Cyclic GMP and 8 Br-cyclic GMP caused a small rise in renin secretion in some experiments but this effect was independent of the dose and its physiological significance is uncertain. 4. Theophylline (10(-6) to 10(14) M) caused a significant elevation in renin secretion which was not blocked by (+)-propranolol. Theophylline with cyclic AMP or cyclic GMP did not produce an amplified effect. 5. Despite previous suggestions that cyclic AMP stimulated renin secretion, this could not be confirmed in the present preparation. Since there is no evidence that cyclic AMP or cyclic GMP (or their derivatives, dibutyryl cyclic AMP and 8 Br-cyclic GMP) enter the cells, it will be necessary to study their activity in isolated juxtaglomerular cells to define a possible role.

摘要

在以克雷布斯葡聚糖溶液灌注的大鼠离体肾脏中，研究了环磷酸腺苷（cAMP）、环磷酸鸟苷（cGMP）和茶碱对肾素分泌的影响。2. 无论是cAMP（10⁻⁶至10⁻⁴M）还是二丁酰环磷腺苷（10⁻⁵M）均未使肾素分泌增加。3. 在某些实验中，cGMP和8-溴环磷鸟苷使肾素分泌略有升高，但这种作用与剂量无关，其生理意义尚不确定。4. 茶碱（10⁻⁶至10⁻⁴M）使肾素分泌显著升高，且不受（+）-普萘洛尔的阻断。茶碱与cAMP或cGMP联合使用未产生增强作用。5. 尽管之前有观点认为cAMP刺激肾素分泌，但在本实验制剂中未能得到证实。由于没有证据表明cAMP或cGMP（或其衍生物，二丁酰环磷腺苷和8-溴环磷鸟苷）进入细胞，因此有必要在分离的球旁细胞中研究它们的活性，以确定其可能的作用。