Ates Burhan, Dogru M Ilker, Gul Mehmet, Erdogan Ali, Dogru Arzu Kocagun, Yilmaz Ismet, Yurekli Muhittin, Esrefoglu Mukaddes
Department of Chemistry, Inonu University, Malatya, 44280, Turkey.
Fundam Clin Pharmacol. 2006 Jun;20(3):283-9. doi: 10.1111/j.1472-8206.2006.00402.x.
Cold exposure can induce a form of environmental stress. Cold stress (CS) alters homeostasis, results in the creation of reactive oxygen species and leads to alterations in the antioxidant defense system. The caffeic acid phenethyl ester (CAPE), an active component of propolis, has an antioxidant capacity. We investigated the effect of CS on oxidative stress and antioxidant defense system and the possible protective effect of CAPE in rat liver tissue. Twenty-four female Wistar Albino rats were divided into four groups: Control, CAPE-treated, CS, and CAPE-treated CS (CS + CAPE) group. Catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) activities and total glutathione (GSH) and malondialdehyde (MDA) levels were measured. In addition, histological changes in liver tissue were examined by light microscopy. SOD, CAT and GSH-Px activities and total GSH level were significantly declined in the CS group. In the CS + CAPE group, the activities of these three enzymes and GSH level significantly raised with regard to the CS group. MDA levels increased in the CS group and decreased in the CS + CAPE group. The tissues of the CS group showed some histopathological changes such as necrosis, hepatocyte degeneration, sinusoidal dilatation, hemorrhage and vascular congestion and dilatation. In the CS + CAPE group, the histopathological evidence of hepatic damage was markedly reduced. Histological parameters were consistent with biochemical parameters. In this study, CS increased oxidative stress in liver tissue. CAPE regulated antioxidant enzymes, inhibited lipid peroxidation and reduced hepatic damage.
寒冷暴露可诱发一种环境应激形式。冷应激（CS）会改变体内平衡，导致活性氧的产生，并引起抗氧化防御系统的改变。咖啡酸苯乙酯（CAPE）是蜂胶的一种活性成分，具有抗氧化能力。我们研究了冷应激对大鼠肝组织氧化应激和抗氧化防御系统的影响以及CAPE可能的保护作用。将24只雌性Wistar白化大鼠分为四组：对照组、CAPE处理组、冷应激组和CAPE处理冷应激组（CS + CAPE组）。测定了过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）活性以及总谷胱甘肽（GSH）和丙二醛（MDA）水平。此外，通过光学显微镜检查肝组织的组织学变化。冷应激组中SOD、CAT和GSH-Px活性以及总GSH水平显著下降。在CS + CAPE组中，这三种酶的活性和GSH水平相对于冷应激组显著升高。冷应激组中MDA水平升高，而CS + CAPE组中MDA水平降低。冷应激组的组织出现了一些组织病理学变化，如坏死、肝细胞变性、窦状隙扩张、出血以及血管充血和扩张。在CS + CAPE组中，肝损伤的组织病理学证据明显减少。组织学参数与生化参数一致。在本研究中，冷应激增加了肝组织中的氧化应激。CAPE调节抗氧化酶，抑制脂质过氧化并减轻肝损伤。
