Jiménez A, López-González M A, Solano F, Chadli A, Delgado F, Pintado E, Lucas M
Molecular Biology Service, Virgen Macarena University Hospital, Sevilla, Spain.
Pediatr Allergy Immunol. 2006 May;17(3):218-20. doi: 10.1111/j.1399-3038.2006.00388.x.
We analyzed the hypomethylation of DNA and the sensitivity to apoptosis of tonsillar cells and peripheral blood lymphocytes (PBL) in twenty children with either recurrent tonsillitis (RT) or tonsillar hypertrophy (TH). We found no significant differences in DNA methylation of PBL obtained from RT and TH groups. Hypomethylation of DNA extracted from tonsillar tissue was higher in TH than in RT and was associated with lower spontaneous and thapsigargin-induced apoptosis. By contrast, RT showed a low level of DNA hypomethylation and was associated with high sensitivity to spontaneous and thapsigargin-induced apoptosis.
我们分析了20名患有复发性扁桃体炎（RT）或扁桃体肥大（TH）的儿童的扁桃体细胞和外周血淋巴细胞（PBL）的DNA低甲基化情况以及对细胞凋亡的敏感性。我们发现，从RT组和TH组获得的PBL的DNA甲基化没有显著差异。从扁桃体组织中提取的DNA的低甲基化在TH组中高于RT组，并且与较低的自发凋亡和毒胡萝卜素诱导的凋亡相关。相比之下，RT组显示出低水平的DNA低甲基化，并与对自发凋亡和毒胡萝卜素诱导的凋亡的高敏感性相关。
