Iimura Sae, Iwahashi Hideo
Department of Chemistry, Wakayama Medical University, 811-1 Kimiidera, Wakayama 641-8509.
J Biochem. 2006 Apr;139(4):671-6. doi: 10.1093/jb/mvj082.
The effects of cigarette smoke extract on radical formation were examined in reaction mixtures containing 13-hydroperoxide octadecadienoic acid (13-HPODE), FeCl3, cigarette smoke extract, ethylenediaminetetraacetic acid (EDTA), alpha-(4-pyridyl-1-oxide)-N-tert-butylnitrone (4-POBN), and phosphate buffer (pH 7.4). Cigarette smoke extract enhanced the formation of both 7-carboxyheptyl and pentyl radicals in the reaction. Ferric ions were reduced in the reaction mixture, suggesting that cigarette smoke extract enhances the formation of 7-carboxyheptyl and pentyl radicals by reducing ferric irons. Although there is a large body of evidence supporting the involvement of radicals such as the semiquinone radical, hydroxyl radical, superoxide radical, nitric oxide radicals in smoking-related diseases, the enhancement by cigarette smoke of lipid-derived radical formation, which we first report here, may be one of the other causes of smoking-related diseases.
在含有13 - 氢过氧化十八碳二烯酸（13 - HPODE）、三氯化铁、香烟烟雾提取物、乙二胺四乙酸（EDTA）、α -（4 - 吡啶 - 1 - 氧化物）- N - 叔丁基硝酮（4 - POBN）和磷酸盐缓冲液（pH 7.4）的反应混合物中，研究了香烟烟雾提取物对自由基形成的影响。香烟烟雾提取物增强了反应中7 - 羧基庚基和戊基自由基的形成。反应混合物中的铁离子被还原，这表明香烟烟雾提取物通过还原铁离子增强了7 - 羧基庚基和戊基自由基的形成。虽然有大量证据支持半醌自由基、羟基自由基、超氧自由基、一氧化氮自由基等自由基参与与吸烟相关的疾病，但我们在此首次报道的香烟烟雾对脂质衍生自由基形成的增强作用可能是与吸烟相关疾病的其他原因之一。
