Iwahashi Hideo
Department of Chemistry, Wakayama Medical University, Wakayama, Japan.
Methods Mol Biol. 2008;477:65-73. doi: 10.1007/978-1-60327-517-0_6.
High performance liquid chromatography/electron spin resonance/mass spectrometry (HPLC/ EPR/MS) analyses of radicals is performed for the reaction mixture of 13-hydroperoxy-(9Z,11E)-octadeca-9,11-dienoic acid (13ZE-OOH) with ferrous ions under an aerobic condition, or an anaerobic condition. Radicals are identified from 13ZE-OOH by using high performance liquid chromatography/electron spin resonance spectrometry (HPLC/EPR) and HPLC/EPR/MS. The pentyl radical and isomers of epoxylinoleic acid radicals from 13ZE-OOH are identified under an anaerobic condition and the 7-carboxyheptyl radical and pentyl radical from 13ZE-OOH under an aerobic condition. These results suggest that the formation of the respective radical species depends to a great extent on oxygen concentration in the reaction mixtures.
在有氧或无氧条件下，对13 - 氢过氧 -（9Z，11E）- 十八碳 - 9,11 - 二烯酸（13ZE - OOH）与亚铁离子的反应混合物进行自由基的高效液相色谱/电子自旋共振/质谱（HPLC/ EPR/MS）分析。通过高效液相色谱/电子自旋共振光谱法（HPLC/EPR）和HPLC/EPR/MS从13ZE - OOH中鉴定自由基。在无氧条件下鉴定出13ZE - OOH产生的戊基自由基和环氧亚油酸自由基的异构体，在有氧条件下鉴定出13ZE - OOH产生的7 - 羧基庚基自由基和戊基自由基。这些结果表明，各自自由基物种的形成在很大程度上取决于反应混合物中的氧气浓度。
