Ando Tomoaki, Suzuki Hiromichi, Nishimura Seiichiro, Tanaka Terumichi, Hiraishi Akira, Kikuchi Yo
Division of Bioscience and Biotechnology, Department of Ecological Engineering, Toyohashi University of Technology, Tempaku-cho, Toyohashi, Aichi 441-8580.
J Biochem. 2006 Apr;139(4):805-11. doi: 10.1093/jb/mvj091.
The marine photosynthetic bacterium Rhodovulum sulfidophilum produces extracellular nucleic acids that are involved in its flocculation. These were found to be produced concomitantly with cell growth. The RNA fraction of these extracellular nucleic acids was subjected to cDNA analysis by applying a micro RNA cloning method and found to contain mainly fully mature-sized tRNAs and fragments of 16S and 23S rRNAs. Analyses of modified bases and genes of the RNAs revealed no structural difference between the intracellular and extracellular RNAs. This is the first report of structural analyses of bacterial extracellular RNAs.
海洋光合细菌嗜硫小红卵菌产生参与其絮凝作用的细胞外核酸。这些核酸被发现与细胞生长同时产生。通过应用微小RNA克隆方法对这些细胞外核酸的RNA部分进行了cDNA分析，发现其主要包含完全成熟大小的tRNA以及16S和23S rRNA的片段。对这些RNA的修饰碱基和基因分析表明，细胞内和细胞外RNA之间没有结构差异。这是关于细菌细胞外RNA结构分析的首次报道。
