Kong Fanrong, Brown Mitchell, Sabananthan Archcna, Zeng Xianyu, Gilbert Gwendolyn L
Centre for Infectious Diseases and Microbiology, Institute of Clinical Pathology and Medical Research, Westmead, New South Wales, Australia.
J Clin Microbiol. 2006 May;44(5):1887-91. doi: 10.1128/JCM.44.5.1887-1891.2006.
We developed a multiplex PCR-based reverse line blot assay to identify 23 pneumococcal serotypes represented in the polysaccharide vaccine, using 334 well-characterized isolates, representing all 90 serotypes, and 268 "unknowns." The assay identified all target serotypes, but 11, which cross-react with 1 to 4 nonvaccine serotypes, could be distinguished using serotype-specific antisera.
我们开发了一种基于多重PCR的反向线印迹检测方法,以鉴定多糖疫苗中所包含的23种肺炎球菌血清型,该检测使用了334株特征明确的分离株(代表所有90种血清型)以及268株“未知”菌株。该检测方法能够鉴定出所有目标血清型,但有11种血清型与1至4种非疫苗血清型发生交叉反应,可使用血清型特异性抗血清加以区分。