O'halloran Damien M, Cafferkey Mary T
Epidemiology and Molecular Biology Unit, The Children's University Hospital, Temple Street, Dublin 1, Ireland.
J Clin Microbiol. 2005 Jul;43(7):3487-90. doi: 10.1128/JCM.43.7.3487-3490.2005.
Here we describe a Streptococcus pneumoniae one-step multiplex PCR assay which identifies by amplicon size the seven capsular polysaccharide serotypes targeted by the 7-valent conjugate vaccine. The multiplex PCR assay was used to blindly assay clinical isolates recovered during 1999 in the Republic of Ireland from cases of invasive disease whose serotypes were previously determined by classical methods.
在此,我们描述了一种肺炎链球菌一步多重聚合酶链反应(PCR)检测方法,该方法通过扩增子大小来鉴定7价结合疫苗所针对的7种荚膜多糖血清型。该多重PCR检测方法被用于对1999年在爱尔兰共和国从侵袭性疾病病例中分离出的临床菌株进行盲测,这些菌株的血清型此前已通过经典方法确定。