Zoulim Fabien
INSERM Unit 271, Institut Universitaire de France, Lyon, France.
Semin Liver Dis. 2006 May;26(2):171-80. doi: 10.1055/s-2006-939759.
Hepatitis B virus resistance to antivirals is conferred by the selection of polymerase gene mutants with reduced susceptibility to nucleoside analogues. Because of the increasing number of resistant mutants to the different available drugs, several phenotypic assays have been developed to provide cross-resistance data to guide clinicians in the management of therapy. Their possible use in the routine follow-up of antiviral treatment is also discussed. The different in vitro models for the study of HBV resistance include enzymatic and cell-based assays. Their relevance for the analysis of viral fitness, drug susceptibility, and cross-resistance is also reviewed.
乙型肝炎病毒对抗病毒药物的耐药性是通过选择对核苷类似物敏感性降低的聚合酶基因突变体产生的。由于对不同现有药物耐药的突变体数量不断增加，已开发了几种表型检测方法，以提供交叉耐药数据，指导临床医生进行治疗管理。还讨论了它们在抗病毒治疗常规随访中的可能用途。用于研究乙肝病毒耐药性的不同体外模型包括酶法检测和基于细胞的检测。还综述了它们在分析病毒适应性、药物敏感性和交叉耐药性方面的相关性。
