Sullebarger J T, Fan T H, Torres F, Liang C S
Department of Medicine, University of Rochester Medical Center, NY 14642.
Eur J Pharmacol. 1991 Nov 26;205(2):165-9. doi: 10.1016/0014-2999(91)90815-8.
To study myocardial beta-adrenoceptor internalization in heart failure, we measured beta-adrenoceptor density in the particulate, light vesicle and supernatant fractions of ventricular tissue of dogs with experimental right ventricular failure and sham-operated dogs. Tissue was fractionated by centrifugation, and beta-adrenoceptors were measured by [125I]iodocyanopindolol binding. Compared to sham-operated controls, beta-adrenoceptors were reduced in all fractions of right ventricular tissue from heart failure animals. Thus, the decreased surface (particulate fraction) receptors observed cannot be explained by internalization alone, and must be associated with altered receptor synthesis or degradation.
为研究心力衰竭时心肌β-肾上腺素能受体的内化，我们测量了实验性右心室衰竭犬和假手术犬心室组织的微粒体、轻囊泡和上清液组分中的β-肾上腺素能受体密度。通过离心对组织进行分级分离，并用[125I]碘氰吲哚洛尔结合法测量β-肾上腺素能受体。与假手术对照组相比，心力衰竭动物右心室组织的所有组分中的β-肾上腺素能受体均减少。因此，观察到的表面（微粒体组分）受体减少不能仅用内化来解释，而必定与受体合成或降解的改变有关。
