Enatsu Sotarou, Iwasaki Akinori, Shirakusa Takayuki, Hamasaki Makoto, Nabeshima Kazuki, Iwasaki Hiroshi, Kuroki Motomu, Kuroki Masahide
Second Department of Surgery, Faculty of Medicine, Fukuoka University, 7-45-1, Nanakuma, Jonan-ku, Fukuoka 814-0180, Japan.
Eur J Cardiothorac Surg. 2006 Jun;29(6):891-5. doi: 10.1016/j.ejcts.2006.03.027. Epub 2006 May 3.
To analyze the prognostic value of hypoxia-inducible factor-1 (HIF-1) alpha expression and its correlation with clinicopathologic variables and the expression of vascular endothelial growth factor-A and -C in patients with lung adenocarcinomas of small size.
The expression of hypoxia-inducible factor-1 alpha was immunohistochemically determined in 78 cases of small-sized adenocarcinoma (maximum dimension < or = 2 cm) using antibody against a recombinant protein corresponding to amino acids 575-780 of hypoxia-inducible factor-1 alpha. Data regarding patient survival, clinicopathologic factors, and immunohistochemical studies of vascular endothelial growth factors were also collected.
Strong expression of hypoxia-inducible factor-1 alpha was observed in 23 of 78 cases; absent or minimal expression was found in the localized bronchioloalveolar carcinomas. Strong expression of hypoxia-inducible factor-1 alpha was significantly higher in cases with vascular invasion, lymph node involvement, and vascular endothelial growth factor-A expression. The 5-year survival rate was 63.2% if expression of hypoxia-inducible factor-1 alpha was strong and 85.1% if expression was weak (p < 0.05).
Immunohistochemical staining of HIF-1 alpha, along with examination of metastatic potential via vascular pathways, may be valid defining a subpopulation of patients with small-sized adenocarcinoma of the lung whose tumors have aggressive angiogenesis potential.
分析低氧诱导因子-1（HIF-1）α表达在小尺寸肺腺癌患者中的预后价值，及其与临床病理变量以及血管内皮生长因子-A和-C表达的相关性。
采用针对与低氧诱导因子-1α氨基酸575 - 780对应的重组蛋白的抗体，通过免疫组织化学方法检测78例小尺寸腺癌（最大直径≤2 cm）中低氧诱导因子-1α的表达。还收集了患者生存、临床病理因素以及血管内皮生长因子免疫组织化学研究的数据。
78例中有23例观察到低氧诱导因子-1α强表达；在局限性细支气管肺泡癌中发现无表达或低表达。低氧诱导因子-1α强表达在有血管侵犯、淋巴结受累和血管内皮生长因子-A表达的病例中显著更高。如果低氧诱导因子-1α表达强，5年生存率为63.2%，如果表达弱则为85.1%（p<0.05）。
HIF-1α免疫组织化学染色，以及通过血管途径检查转移潜能，可能有效地定义一小部分具有侵袭性血管生成潜能的小尺寸肺腺癌患者亚群。
