Skop B, Drózdz M, Zak I
Chair of Biochemistry, Silesian Academy of Medicine, Katowice, Poland.
Acta Biochim Pol. 1991;38(2):219-27.
Proteoglycans (PG) from normal and atherosclerotic rabbits aortas were extracted with 4 M guanidine hydrochloride and digested with collagenase in the presence of protease inhibitors. The contents of uronic acid and hexosamine from PG fractions purified by isopycnic CsCl gradient ultracentrifugation under associative and dissociative conditions were significantly higher in the atherosclerotic aortas (up to 40%) than in the control tissue. The uronic acid/protein ratio increased from 0.7 to 1.3 in the monomers PG fraction of atherosclerotic aortas. Chromatographic separation and electrophoretic analysis of PG monomers indicated the presence of three different subfractions PGI, PGII and PGIII in both groups of animals. The uronic acid/protein ratio in PGI from experimental aorta was increased whereas this ratio in PGIII was decreased compared to contrast tissue. The observed increase of sugar component in the core proteins suggests their over glycosylation.
从正常和动脉粥样硬化兔主动脉中提取蛋白聚糖（PG），用4M盐酸胍提取，并在蛋白酶抑制剂存在的情况下用胶原酶消化。在缔合和解离条件下，通过等密度CsCl梯度超速离心纯化的PG组分中，动脉粥样硬化主动脉中的糖醛酸和己糖胺含量显著高于对照组织（高达40%）。动脉粥样硬化主动脉的单体PG组分中，糖醛酸/蛋白质比值从0.7增加到1.3。PG单体的色谱分离和电泳分析表明，两组动物中均存在三种不同的亚组分PGI、PGII和PGIII。与对照组织相比，实验主动脉PGI中的糖醛酸/蛋白质比值增加，而PGIII中的该比值降低。核心蛋白中糖成分的增加表明它们存在过度糖基化。
