Zuo Zehua, Zhao Min, Liu Juan, Gao Guifang, Wu Xinxing
Wuhan University, Wuhan, China.
Tumour Biol. 2006;27(4):221-6. doi: 10.1159/000093057. Epub 2006 May 2.
Our previous study has suggested thatthe bladder cancer-associated protein gene (BLCAP) was among the differentially expressed genes in cervical cancer. We confirm here that BLCAP is expressed in all noncancerous cervical tissues (10/10), but it is greatly lost in primary cervical cancer tissue (31/39). In order to further investigate the functional roles of BLCAP, we stably transfected BLCAP cDNA into HeLa cells. The HeLa cells expressing BLCAP show reduced cell growth and clone genicity compared to the vector-transfected cognate cells. BLCAP expression in HeLa cells leads to growth arrest and significantly enhanced apoptosis in vitro and reduced tumor formation in vivo. Thus, BLCAP might be a potential tumor suppressor gene in cervical carcinoma.
我们之前的研究表明，膀胱癌相关蛋白基因（BLCAP）是宫颈癌中差异表达的基因之一。我们在此证实，BLCAP在所有非癌性宫颈组织中均有表达（10/10），但在原发性宫颈癌组织中其表达大量缺失（31/39）。为了进一步研究BLCAP的功能作用，我们将BLCAP cDNA稳定转染至HeLa细胞。与载体转染的同源细胞相比，表达BLCAP的HeLa细胞显示出细胞生长和克隆形成能力降低。HeLa细胞中BLCAP的表达导致体外生长停滞、凋亡显著增强以及体内肿瘤形成减少。因此，BLCAP可能是宫颈癌中的一个潜在抑癌基因。
