一种由三磷酸腺苷（ATP）驱动的氯离子泵调节大鼠海马神经元中的氯离子浓度。
An ATP-driven Cl- pump regulates Cl- concentrations in rat hippocampal neurons.
作者信息
Inoue M, Hara M, Zeng X T, Hirose T, Ohnishi S, Yasukura T, Uriu T, Omori K, Minato A, Inagaki C
机构信息
Department of Pharmacology, Kansai Medical University, Osaka, Japan.
出版信息
Neurosci Lett. 1991 Dec 16;134(1):75-8. doi: 10.1016/0304-3940(91)90512-r.
To investigate the role of Cl(-)-stimulated Mg(2+)-ATPase (Cl(-)-ATPase) in neurons, we examined the effects of ethacrynic acid (0.3 mM), which completely inhibits Cl(-)-ATPase on the intracellular Cl- concentrations of cultured rat hippocampal neurons, using Cl(-)-sensitive fluorescent probes. Ethacrynic acid and ATP consuming treatment increased the intracellular Cl- concentration, but elevation of the extracellular K+ concentration up to 10 mM, inhibition of Na+/K(+)-ATPase, or dissolution of H+ gradients had no effect. Furosemide (0.1 mM), an inhibitor of Na+/K+/Cl- co-transport, decreased the intracellular Cl- concentrations. These results indicate that an ethacrynic acid-sensitive and ATP-driven Cl- pump functions to reduce intraneural Cl- concentrations.
为了研究氯离子刺激的镁离子 - 三磷酸腺苷酶（Cl(-)-ATPase）在神经元中的作用，我们使用对氯离子敏感的荧光探针，检测了完全抑制Cl(-)-ATPase的依他尼酸（0.3 mM）对培养的大鼠海马神经元细胞内氯离子浓度的影响。依他尼酸和消耗三磷酸腺苷（ATP）的处理增加了细胞内氯离子浓度，但将细胞外钾离子浓度提高到10 mM、抑制钠钾ATP酶或消除氢离子梯度均无影响。呋塞米（0.1 mM），一种钠钾氯共转运抑制剂，降低了细胞内氯离子浓度。这些结果表明，一种对依他尼酸敏感且由ATP驱动的氯离子泵发挥作用以降低神经内氯离子浓度。
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