Ishibashi Hiroshi, Hirano Masashi, Matsumura Naomi, Watanabe Naoko, Takao Yuji, Arizono Koji
Center for Marine Environmental Studies (CMES), Ehime University, Bunkyo-cho 2-5, Matsuyama 790-8577, Japan.
Chemosphere. 2006 Nov;65(6):1019-26. doi: 10.1016/j.chemosphere.2006.03.034. Epub 2006 May 4.
The objective of this study was to investigate the reproductive success (fertility and fecundity) of medaka (Oryzias latipes) exposed to 4-nonylphenol (4-NP) in water and the viability of their F(1) offspring. In addition, we measured the bioconcentration of 4-NP in eggs. After a 21-d exposure to 100microg/l 4-NP, medaka showed reduced egg production and fertility. Hepatic vitellogenin levels were increased significantly in males treated with 10, 50 and 100microg/l of 4-NP. In the F(1) generations, the hatchability and time to hatching of embryos in the 100microg/l treatment group were adversely affected, and 2-7microg 4-NP/g egg was found in spawned eggs (the bioconcentration factor: 30-100). These results indicate that (1) 4-NP adversely affected the reproduction (fecundity and fertility) of adult medaka, (2) 4-NP accumulated in eggs through maternal transfer and (3) these levels of 4-NP were associated with adverse effects in the F(1) offspring.
本研究的目的是调查暴露于水中4-壬基酚（4-NP）的青鳉（Oryzias latipes）的繁殖成功率（生育力和繁殖力）及其F1代后代的活力。此外，我们还测量了卵中4-NP的生物富集情况。在暴露于100μg/l 4-NP 21天后，青鳉的产卵量和生育力降低。用10、50和100μg/l的4-NP处理的雄性青鳉肝脏卵黄蛋白原水平显著升高。在F1代中，100μg/l处理组胚胎的孵化率和孵化时间受到不利影响，在产出的卵中发现2-7μg 4-NP/g卵（生物富集系数：30-100）。这些结果表明：（1）4-NP对成年青鳉的繁殖（繁殖力和生育力）产生不利影响；（2）4-NP通过母体转移在卵中积累；（3）这些4-NP水平与F1代后代的不良影响有关。
