Rehan Mohd, Younus Hina
Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India.
Int J Biol Macromol. 2006 May 30;38(3-5):289-95. doi: 10.1016/j.ijbiomac.2006.03.023. Epub 2006 Mar 29.
Effect of six organic solvents-methanol, ethanol, propanol, dimethyl sulphoxide (DMSO), N,N-dimethyl formamide (DMF), and glycerol on the conformation and interaction of catalase and anticatalase antibodies were studied with the aim of identifying the solvents in which antigen-antibody interactions are strong. The antigen binding activity of the antibodies in the various organic solvents increased in the following order: ethanol<methanol<no organic solvent<propanol<DMSO<DMF<glycerol. The structure of both the antibody and the antigen molecule was affected significantly in 40% concentration of the organic solvents used in this study. Catalase activity was inhibited in DMSO. However, the enzyme was activated in DMF upto about 50% of its concentration.
研究了六种有机溶剂——甲醇、乙醇、丙醇、二甲基亚砜（DMSO）、N，N-二甲基甲酰胺（DMF）和甘油对过氧化氢酶和抗过氧化氢酶抗体的构象及相互作用的影响，目的是确定抗原-抗体相互作用较强的溶剂。抗体在各种有机溶剂中的抗原结合活性按以下顺序增加：乙醇＜甲醇＜无有机溶剂＜丙醇＜DMSO＜DMF＜甘油。在本研究使用的40%浓度的有机溶剂中，抗体和抗原分子的结构均受到显著影响。过氧化氢酶活性在DMSO中受到抑制。然而，该酶在DMF中被激活，其浓度可达约50%。
