Yanglin Pan, Lina Zhao, Zhiguo Liu, Na Liu, Haifeng Jin, Guoyun Zhang, Jie Liang, Jun Wang, Tao Lin, Li Sun, Taidong Qiao, Jianhong Wang, Daiming Fan
State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi Province, PR China.
Cancer Lett. 2007 Feb 8;246(1-2):129-38. doi: 10.1016/j.canlet.2006.02.010. Epub 2006 May 4.
It is important to identify the differentially expressed gene in gastric cancer for elucidating the molecular mechanisms of tumorigenesis of stomach. Here, 38 genes differentially expressed genes between gastric cancer and normal gastric mucosa by in silico approaches. A potassium channel protein KCNE2, identified as a down-regulated gene in gastric cancer, was chosen for further study. We investigated the expression of KCNE2 in gastric cancer tissues and cell lines and examined the effect of KCNE2 on proliferation of gastric cancer. The expression of KCNE2 was markedly down-regulated in gastric cancer tissues and cell lines. Forced overexpression of KCNE2 suppressed the growth of SGC7901 cells and cell cycle progression significantly, which might be related to the down-regulation of Cyclin D1. KCNE2 also inhibited SGC7901 cell growth in soft agar and its tumorigenicity in nude mice. Taken together, our work showed that in silico analysis approaches could be used to identify cancer-related genes effectively. KCNE2, as a novel down-regulated gene in gastric cancer, suppressed cell proliferation and tumorigenesis of stomach.
识别胃癌中差异表达的基因对于阐明胃癌发生的分子机制很重要。在此，通过计算机分析方法鉴定出38个在胃癌和正常胃黏膜之间差异表达的基因。选择一种钾通道蛋白KCNE2，其被鉴定为胃癌中下调的基因，进行进一步研究。我们研究了KCNE2在胃癌组织和细胞系中的表达，并检测了KCNE2对胃癌增殖的影响。KCNE2的表达在胃癌组织和细胞系中明显下调。KCNE2的强制过表达显著抑制了SGC7901细胞的生长和细胞周期进程，这可能与细胞周期蛋白D1的下调有关。KCNE2还抑制了SGC7901细胞在软琼脂中的生长及其在裸鼠中的致瘤性。综上所述，我们的工作表明计算机分析方法可有效用于鉴定癌症相关基因。KCNE2作为胃癌中一个新的下调基因，抑制了胃细胞的增殖和肿瘤发生。
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