[甲苯二异氰酸酯（TDI）所致职业性哮喘的实验病因学研究]

[An experimental etiological study of toluene diisocyanate (TDI) induced occupational asthma].

作者信息

Liu J Y

机构信息

Research Center of Occupational Medicine, Third Hospital of Beijing Medical University.

出版信息

Zhonghua Yu Fang Yi Xue Za Zhi. 1991 Nov;25(6):347-50.

PMID:1667862

Abstract

Thirteen guinea pigs were immunized with TDI-BSA conjugates employing CFA or AHG adjuvant or only adjuvant as a control via intraperitoneal route. After 5-8 weeks, 30.8% of the sensitized animals experienced asthma attacks after a TDI-HSA challenge. The dynamic airway pulmonary ventilation imaging with Tc-99m-DTPA showed an accumulation of radioactivity. The radioactivity ratio of trachea or bronchus/lung was increased 6.46-29 times in contrast with before challenge. A specific IgE and IgG type antibodies were developed in 87.5% of TDI-BSA/CFA sensitized animals and 40% of TDI-BSA/AHG sensitized animals by passive cutaneous anaphylaxis (PCA). Results of these suggested that allergic response play an important role in the mechanism of TDI-induced asthma.

摘要

通过腹腔途径，用含有完全弗氏佐剂（CFA）或抗人球蛋白（AHG）佐剂的甲苯二异氰酸酯-牛血清白蛋白（TDI-BSA）偶联物对13只豚鼠进行免疫，或仅用佐剂作为对照。5-8周后，30.8%的致敏动物在接受TDI-人血清白蛋白（TDI-HSA）激发后出现哮喘发作。用锝-99m-二乙三胺五乙酸（Tc-99m-DTPA）进行的动态气道肺通气成像显示有放射性聚集。与激发前相比，气管或支气管/肺的放射性比值增加了6.46-29倍。通过被动皮肤过敏反应（PCA），在87.5%的TDI-BSA/CFA致敏动物和40%的TDI-BSA/AHG致敏动物中产生了特异性IgE和IgG型抗体。这些结果表明，过敏反应在TDI诱发哮喘的机制中起重要作用。