一种用于呼吸测量研究的用户友好型基于个人计算机的数据采集与分析系统。

A user-friendly PC-based data acquisition and analysis system for respirometric investigations.

作者信息

Kopustinskas Audris, Adaskevicius Rimas, Krusinskas Andrius, Kopustinskiene Dalia M, Liobikas Julius, Toleikis Adolfas

机构信息

Faculty of Electrical and Control Engineering, Kaunas University of Technology, Studentu st. 50-303, LT-51368 Kaunas, Lithuania.

出版信息

Comput Methods Programs Biomed. 2006 Jun;82(3):231-7. doi: 10.1016/j.cmpb.2006.02.012. Epub 2006 May 6.

DOI:10.1016/j.cmpb.2006.02.012

PMID:16678932

Abstract

We have developed an easy-to-use computer-based system for recording, displaying, storing and analyzing signals generated by Clark-type oxygen electrodes. A user-friendly interface of Windows-based program BioMed significantly increases the productivity of investigations. It allows to process, control, present and archive the experimental data in real time. A 12-bit analog-to-digital-converter, analog and digital filters, a possibility to zoom the obtained respiratory curves and calculation of the respiration rates by a linear regression method increase the resolution of the estimated oxygen consumption rates. The new system enables to register even small changes, such as 3-5 ngatoms O/min, in respiration rates of biological objects -- enzymes, mitochondria and permeabilized muscle fibers. The system has been developed and is regularly used for the respirometric investigations at the Laboratory of Biochemistry, Institute for Biomedical Research, Kaunas University of Medicine.

摘要

我们开发了一种易于使用的基于计算机的系统，用于记录、显示、存储和分析克拉克型氧电极产生的信号。基于Windows的程序BioMed的用户友好界面显著提高了研究效率。它允许实时处理、控制、呈现和存档实验数据。12位模数转换器、模拟和数字滤波器、缩放所获得的呼吸曲线的可能性以及通过线性回归方法计算呼吸率，提高了估计的氧消耗率的分辨率。新系统能够记录生物对象（酶、线粒体和透化肌纤维）呼吸率中即使很小的变化，例如3 - 5纳克原子氧/分钟。该系统已开发完成，并在考纳斯医科大学医学院生物医学研究所生物化学实验室定期用于呼吸测量研究。