Lü Min, Xia Bing, Li Jin, Ye Mei, Zhang Xiaolian, Tan Qinquan
Department of Internal Medicine and Geriatrics, and Research Center of Digestive Diseases, Zhongnan Hospital, PR China.
Clin Immunol. 2006 Aug;120(2):199-204. doi: 10.1016/j.clim.2006.03.001. Epub 2006 May 5.
The MHC class I-related molecules A and B (MICA and MICB) are stress-inducible cell surface antigens that are recognized by immunocytes bearing the receptor NKG2D, including intestinal epithelial Vdelta1 gammadelta T cells, which may play a role in immunological reaction in intestinal mucosa. The present study was aimed to investigate the association of the microsatellite polymorphisms in the intron 1 of MICB and the MICA-MICB haplotype with the susceptibility to ulcerative colitis (UC) in Chinese population. The microsatellite polymorphisms of MICB were genotyped in unrelated 127 Chinese patients with UC and 193 ethnically matched healthy controls by a semiautomatic fluorescently labeled PCR method. All the subjects were the Chinese with Han nationality. The frequency of MICB-CA18 was significantly higher in UC patients compared with the healthy controls (14.0% vs. 5.8%, P = 0.0016, Pc = 0.024, OR = 2.637, 95%CI: 1.443-4.820) and was increased in the female patients compared with the female healthy controls (18.3% vs. 4.1%, P = 0.0006, Pc = 0.0080, OR = 5.224, 95%CI: 1.940-14.069). Thus, MICB-CA18 is positively associated with UC and female UC patients in Chinese population.
主要组织相容性复合体I类相关分子A和B（MICA和MICB）是应激诱导的细胞表面抗原，可被携带NKG2D受体的免疫细胞识别，包括肠道上皮Vδ1γδT细胞，它们可能在肠道黏膜的免疫反应中发挥作用。本研究旨在探讨中国人群中MICB基因内含子1的微卫星多态性及MICA - MICB单倍型与溃疡性结肠炎（UC）易感性的关系。采用半自动荧光标记PCR方法，对127例无血缘关系的中国UC患者和193例种族匹配的健康对照进行MICB微卫星多态性基因分型。所有受试者均为汉族中国人。与健康对照相比，UC患者中MICB - CA18的频率显著更高（14.0%对5.8%，P = 0.0016，Pc = 0.024，OR = 2.637，95%CI：1.443 - 4.820），且女性患者中该频率高于女性健康对照（18.3%对4.1%，P = 0.0006，Pc = 0.0080，OR = 5.224，95%CI：1.940 - 14.069）。因此，在中国人群中，MICB - CA18与UC及女性UC患者呈正相关。
