Litzlbauer Horst Detlef, Neuhaeuser Christoph, Moell Alexander, Greschus Susanne, Breithecker Andreas, Franke Folker Ernst, Kummer Wolfgang, Rau Wigbert Stephan
Department of Diagnostic Radiology, University of Giessen, Germany.
Am J Physiol Lung Cell Mol Physiol. 2006 Sep;291(3):L535-45. doi: 10.1152/ajplung.00088.2005. Epub 2006 May 5.
We evaluated microfocal X-ray-computed tomography (micro-CT) as a method to visualize lung architecture two and three dimensionally and to obtain morphometric data. Inflated porcine lungs were fixed by formaldehyde ventilation. Tissue samples (8-mm diameter, 10-mm height) were stained with osmium tetroxide, and 400 projection images (1,024 x 1,024 pixel) were obtained. Continuous isometric micro-CT scans (voxel size 9 microm) were acquired to reconstruct two- and three-dimensional images. Tissue samples were sectioned (8-microm thickness) for histological analysis. Alveolar surface density and mean linear intercept were assessed by stereology-based morphometry in micro-CT scans and corresponding histological sections. Furthermore, stereology-based morphometry was compared with morphometric semi-automated micro-CT analysis within the same micro-CT scan. Agreement of methods was assessed by regression and Bland-Altman analysis. Comparing histology with micro-CT, alveolar surface densities (35.4 +/- 2.4 vs. 33.4 +/- 1.9/mm, P < 0.05) showed a correlation (r = 0.72; P = 0.018) with an agreement of 2 +/- 1.6/mm; the mean linear intercept (135.7 +/- 14.5 vs. 135.8 +/- 15 microm) correlated well (r = 0.97; P < 0.0001) with an agreement of -0.1 +/- 3.4 microm. Semi-automated micro-CT analysis resulted in smaller alveolar surface densities (33.4 +/- 1.9 vs. 30.5 +/- 1/mm; P < 0.01) with a correlation (r = 0.70; P = 0.023) and agreement of 2.9 +/- 1.4/mm. Non-destructive micro-CT scanning offers the advantage to visualize the spatial tissue architecture of small lung samples two and three dimensionally.
我们评估了微焦点X射线计算机断层扫描（micro-CT）作为一种二维和三维可视化肺结构并获取形态计量学数据的方法。通过甲醛通气固定充气的猪肺。将组织样本（直径8毫米，高10毫米）用四氧化锇染色，并获得400张投影图像（1024×1024像素）。进行连续等距微CT扫描（体素大小9微米）以重建二维和三维图像。将组织样本切片（厚度8微米）用于组织学分析。在微CT扫描和相应的组织学切片中，通过基于体视学的形态计量学评估肺泡表面密度和平均线性截距。此外，在同一微CT扫描中，将基于体视学的形态计量学与形态计量学半自动微CT分析进行比较。通过回归分析和布兰德-奥特曼分析评估方法的一致性。将组织学与微CT进行比较，肺泡表面密度（35.4±2.4对33.4±1.9/mm，P<0.05）显示出相关性（r = 0.72；P = 0.018），一致性为2±1.6/mm；平均线性截距（135.7±14.5对135.8±15微米）相关性良好（r = 0.97；P<0.0001），一致性为-0.1±3.4微米。半自动微CT分析导致肺泡表面密度较小（33.4±1.9对30.5±1/mm；P<0.01），相关性（r = 0.70；P = 0.023）和一致性为2.9±1.4/mm。非破坏性微CT扫描具有二维和三维可视化小肺样本空间组织结构的优势。
