Ottonello L, Dallegri F, Dapino P, Patrone F, Sacchetti C
Department of Internal Medicine, University of Genova Medical School, Italy.
J Clin Lab Immunol. 1991 Jan;34(1):37-40.
Peripheral blood neutrophil polymorphonuclear leukocytes (PMN) from healthy donors were found to inhibit the cytolytic efficiency of interleukin 2 (IL-2)-activated lymphocytes (LAK cells) in a dose-dependent manner. The inhibitory activity of PMN was not merely due to PMN acting as cold alternative targets, PMN ingestion of the label released by target cells or cell overcrowding in test wells. Heat-treated (50 degrees C, 30 min) lysates from PMN maintained their ability to inhibit LAK cell cytotoxicity, whereas PMN supernatants were completely ineffective. Oxidant scavengers (catalase, superoxide, dismutase) did not affect the PMN-mediated inhibition of LAK cell function. The results suggest that PMN contain heat-stable factor(s) able to suppress LAK cytotoxicity and potentially capable of limiting the therapeutic efficacy of IL-2 and/or LAK cells.
研究发现，来自健康供体的外周血中性粒细胞多形核白细胞（PMN）能以剂量依赖的方式抑制白细胞介素2（IL-2）激活的淋巴细胞（LAK细胞）的细胞溶解效率。PMN的抑制活性不仅仅是由于PMN作为冷替代靶标、PMN摄取靶细胞释放的标记物或试验孔中细胞过度拥挤。经热处理（50℃，30分钟）的PMN裂解物仍保持其抑制LAK细胞细胞毒性的能力，而PMN上清液则完全无效。抗氧化剂（过氧化氢酶、超氧化物歧化酶）不影响PMN介导的对LAK细胞功能的抑制。结果表明，PMN含有能够抑制LAK细胞毒性的热稳定因子，并且可能会限制IL-2和/或LAK细胞的治疗效果。
