Stipanuk M H, Dominy J E
Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853, USA.
Amino Acids. 2006 May;30(3):251-6. doi: 10.1007/s00726-005-0288-4. Epub 2006 May 4.
The modeling of whole organism sulfur amino acid flux control has been aided in recent years by advancements in proteomics and mass spectroscopy-based metabolite analysis. The convergence of these two fields and their respective techniques, as demonstrated by a new study using yeast by Lafaye et al., has shown that researchers seeking to model whole cell/organism metabolism should give careful consideration to the relationships connecting enzyme concentration, enzyme activity, substrate concentration, and metabolic flux. In this paper, we outline some of the fundamental concepts for modeling sulfur amino acid metabolism and how they relate to our current understanding of mammalian sulfur amino acid metabolism.
近年来，蛋白质组学和基于质谱的代谢物分析技术的进步推动了对整个生物体硫氨基酸通量控制的建模。正如Lafaye等人利用酵母进行的一项新研究所表明的那样，这两个领域及其各自技术的融合表明，试图对全细胞/生物体代谢进行建模的研究人员应仔细考虑连接酶浓度、酶活性、底物浓度和代谢通量之间的关系。在本文中，我们概述了硫氨基酸代谢建模的一些基本概念，以及它们与我们目前对哺乳动物硫氨基酸代谢的理解之间的关系。
